Golgin-160 promotes cell surface expression of the beta-1 adrenergic receptor

Stuart W. Hicks, Troy A. Horn, J. Michael Mccaffery, David M. Zuckerman, Carolyn E. Machamer

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Golgin-160 is a ubiquitously expressed peripheral Golgi membrane protein that is important for transduction of certain pro-apoptotic signals at the Golgi complex. However, the role of golgin-160 in normal Golgi structure and function is unknown. Here, we show that depletion of golgin-160 using RNA interference (RNAi) does not affect Golgi morphology or constitutive membrane traffic in HeLa cells. However, depletion of golgin-160 leads to significantly decreased cell surface levels of exogenously expressed β1-adrenergic receptor (β1AR), which can be rescued by expression of RNAi-resistant forms of golgin-160. Furthermore, overexpression of golgin-160 leads to higher surface levels of β1AR. Golgin-160 is localized mostly in the cis and medial regions of the Golgi stack by immunoelectron microscopy, suggesting that it does not directly promote incorporation of β1AR into transport vesicles at the trans Golgi network. Golgin-160 interacts with β1AR in vitro, and we mapped the interaction to a region between residues 140 and 257 in the head of golgin-160 and the third intracellular loop of β1AR. Our results support the idea that golgin-160 may promote efficient surface delivery of a subset of cargo molecules.

Original languageEnglish (US)
Pages (from-to)1666-1677
Number of pages12
Issue number12
StatePublished - Dec 2006


  • Golgi
  • Golgin-160
  • PIST
  • Trafficking
  • β1-adrenergic receptor

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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