Golgin-160 promotes cell surface expression of the beta-1 adrenergic receptor

Stuart W. Hicks, Troy A. Horn, J. Michael Mccaffery, David M. Zuckerman, Carolyn E Machamer

Research output: Contribution to journalArticle

Abstract

Golgin-160 is a ubiquitously expressed peripheral Golgi membrane protein that is important for transduction of certain pro-apoptotic signals at the Golgi complex. However, the role of golgin-160 in normal Golgi structure and function is unknown. Here, we show that depletion of golgin-160 using RNA interference (RNAi) does not affect Golgi morphology or constitutive membrane traffic in HeLa cells. However, depletion of golgin-160 leads to significantly decreased cell surface levels of exogenously expressed β1-adrenergic receptor (β1AR), which can be rescued by expression of RNAi-resistant forms of golgin-160. Furthermore, overexpression of golgin-160 leads to higher surface levels of β1AR. Golgin-160 is localized mostly in the cis and medial regions of the Golgi stack by immunoelectron microscopy, suggesting that it does not directly promote incorporation of β1AR into transport vesicles at the trans Golgi network. Golgin-160 interacts with β1AR in vitro, and we mapped the interaction to a region between residues 140 and 257 in the head of golgin-160 and the third intracellular loop of β1AR. Our results support the idea that golgin-160 may promote efficient surface delivery of a subset of cargo molecules.

Original languageEnglish (US)
Pages (from-to)1666-1677
Number of pages12
JournalTraffic
Volume7
Issue number12
DOIs
StatePublished - Dec 2006

Fingerprint

Adrenergic beta-1 Receptors
Adrenergic Receptors
RNA Interference
RNA
trans-Golgi Network
Transport Vesicles
Immunoelectron Microscopy
Golgi Apparatus
HeLa Cells
Microscopic examination
Membrane Proteins
Head
Membranes
Molecules

Keywords

  • β1-adrenergic receptor
  • Golgi
  • Golgin-160
  • PIST
  • Trafficking

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Golgin-160 promotes cell surface expression of the beta-1 adrenergic receptor. / Hicks, Stuart W.; Horn, Troy A.; Mccaffery, J. Michael; Zuckerman, David M.; Machamer, Carolyn E.

In: Traffic, Vol. 7, No. 12, 12.2006, p. 1666-1677.

Research output: Contribution to journalArticle

Hicks, Stuart W. ; Horn, Troy A. ; Mccaffery, J. Michael ; Zuckerman, David M. ; Machamer, Carolyn E. / Golgin-160 promotes cell surface expression of the beta-1 adrenergic receptor. In: Traffic. 2006 ; Vol. 7, No. 12. pp. 1666-1677.
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