Golgin-160 is required for the Golgi membrane sorting of the insulin-responsive glucose transporter GLUT4 in adipocytes

Dumaine Williams, Stuart W. Hicks, Carolyn E Machamer, Jeffrey E. Pessin

Research output: Contribution to journalArticle

Abstract

The peripheral Golgi protein golgin-160 is induced during 3T3L1 adipogenesis and is primarily localized to the Golgi cisternae distinct from the frans-Golgi network (TGN) in a general distribution similar to p115. Small interfering RNA (siRNA)-mediated reduction in golgin-160 protein resulted in an increase accumulation of the insulin-responsive amino peptidase (IRAP) and the insulin-regulated glucose transporter (GLUT4) at the plasma membrane concomitant with enhanced glucose uptake in the basal state. The redistribution of GLUT4 was rescued by expression of a siRNA-resistant golgin-160 cDNA. The basal state accumulation of plasma membrane GLUT4 occurred due to an increased rate of exocytosis without any significant effect on the rate of endocytosis. This GLUT4 trafficking to the plasma membrane in the absence of golgin-160 was independent of TGN/Golgi sorting, because it was no longer inhibited by the expression of a dominant-interfering Golgi-localized, γ-ear-containing ARF-binding protein mutant and displayed reduced binding to the lectin wheat germ agglutinin. Moreover, expression of the amino terminal head domain (amino acids 1-393) had no significant effect on the distribution or insulin-regulated trafficking of GLUT4 or IRAP. In contrast, expression of carboxyl γ helical region (393-1498) inhibited insulin-stimulated GLUT4 and IRAP translocation, but it had no effect on the sorting of constitutive membrane trafficking proteins, the transferrin receptor, or vesicular stomatitis virus G protein. Together, these data demonstrate that golgin-160 plays an important role in directing insulin-regulated trafficking proteins toward the insulin-responsive compartment in adipocytes.

Original languageEnglish (US)
Pages (from-to)5346-5355
Number of pages10
JournalMolecular Biology of the Cell
Volume17
Issue number12
DOIs
StatePublished - Dec 2006

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Glucose Transporter Type 4
Adipocytes
Insulin
Membranes
Peptide Hydrolases
Cell Membrane
Small Interfering RNA
Adipogenesis
Wheat Germ Agglutinins
Transferrin Receptors
Facilitative Glucose Transport Proteins
Exocytosis
Protein Transport
Endocytosis
Lectins
Ear
Carrier Proteins
Membrane Proteins
Proteins
Complementary DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Golgin-160 is required for the Golgi membrane sorting of the insulin-responsive glucose transporter GLUT4 in adipocytes. / Williams, Dumaine; Hicks, Stuart W.; Machamer, Carolyn E; Pessin, Jeffrey E.

In: Molecular Biology of the Cell, Vol. 17, No. 12, 12.2006, p. 5346-5355.

Research output: Contribution to journalArticle

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