TY - JOUR
T1 - G(o), a guanine nucleotide-binding protein
T2 - Immunohistochemical localization in rat brain resembles distribution of second messenger systems
AU - Worley, P. F.
AU - Baraban, J. M.
AU - Van Dop, C.
PY - 1986
Y1 - 1986
N2 - We have localized a guanine nucleotide-binding protein, G(o), in rat brain by immunohistochemistry with a selective polyclonal antiserum to the α39 subunit of G(o). Specific staining is widely distributed, abundant in neuropil, absent from neuronal cell bodies, and displays regional heterogeneity. Staining is enriched in cerebral cortex, particularly the molecular layer, neuropil of the hippocampal formation, striatum, substantia nigra pars reticulata, molecular layer of the cerebellum, substantia gelatinosa of the spinal cord, and posterior pituitary. High density staining in the substantia nigra reflects a G(o)-containing striatonigral pathway since striatal lesions reduce ipsilateral immunostaining in the pars reticulata. Confirming immunostaining, quantitative [32P]ADP-ribosylation of nigral membranes with pertussis toxin indicates a 66% ± 11% (mean ± SEM) reduction of G(o) ipsilateral to striatal lesions. G(o) may be associated with Purkinje cells in the cerebellum since membranes from mutant mice (Nervous), which postnatally lose Purkinje cells, are markedly depleted in pertussis toxin substrate. The localizations of G(o) correspond in many areas with those of protein kinase C, a component of the phosphatidylinositol cycle, suggesting a major role for G(o) in the brain related to regulation of the phosphatidylinositol cycle.
AB - We have localized a guanine nucleotide-binding protein, G(o), in rat brain by immunohistochemistry with a selective polyclonal antiserum to the α39 subunit of G(o). Specific staining is widely distributed, abundant in neuropil, absent from neuronal cell bodies, and displays regional heterogeneity. Staining is enriched in cerebral cortex, particularly the molecular layer, neuropil of the hippocampal formation, striatum, substantia nigra pars reticulata, molecular layer of the cerebellum, substantia gelatinosa of the spinal cord, and posterior pituitary. High density staining in the substantia nigra reflects a G(o)-containing striatonigral pathway since striatal lesions reduce ipsilateral immunostaining in the pars reticulata. Confirming immunostaining, quantitative [32P]ADP-ribosylation of nigral membranes with pertussis toxin indicates a 66% ± 11% (mean ± SEM) reduction of G(o) ipsilateral to striatal lesions. G(o) may be associated with Purkinje cells in the cerebellum since membranes from mutant mice (Nervous), which postnatally lose Purkinje cells, are markedly depleted in pertussis toxin substrate. The localizations of G(o) correspond in many areas with those of protein kinase C, a component of the phosphatidylinositol cycle, suggesting a major role for G(o) in the brain related to regulation of the phosphatidylinositol cycle.
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U2 - 10.1073/pnas.83.12.4561
DO - 10.1073/pnas.83.12.4561
M3 - Article
C2 - 3086888
AN - SCOPUS:0022480713
SN - 0027-8424
VL - 83
SP - 4561
EP - 4565
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -