Glypican-3 expression in wilms tumor and hepatoblastoma

Jeffrey A. Toretsky, Naamah L. Zitomersky, Allen E. Eskenazi, Roger W. Voigt, Eric D. Strauch, Chem Chih Sun, Reed Huber, Steven J. Meltzer, David Schlessinger

Research output: Contribution to journalArticlepeer-review

Abstract

Background Glypican-3 (GPC3) is a heparan sulfate proteoglycan. When it is disrupted, it causes the X-linked gigantism-overgrowth Simpson-Golabi-Behmel syndrome. Its involvement in growth control is consistent with recent reports that it can bind to growth factors, possibly including insulin-like growth factor 2. Further, it has been hypothesized that it may function as a tumor suppressor gene in breast and ovarian carcinomas and mesotheliomas. Patients and Methods RNA and protein were extracted from Wilms tumor and hepatoblastoma tissue samples and GPC3 levels were measured in these extracts by Northern blotting, reverse transcription polymerase chain reaction, and immunoblotting. Results In contrast to published results with carcinomas, high levels of GPC3 expression were found in Wilms tumor and hepatoblastoma. Low or undetectable expressions of this gene were found in normal tissue surrounding the tumor. Conclusions Increased expression of GPC3 in Wilms tumor and hepatoblastoma suggests a growth-promoting or neutral activity for this gene product rather than a growth-suppressive effect.

Original languageEnglish (US)
Pages (from-to)496-499
Number of pages4
JournalAmerican Journal of Pediatric Hematology/Oncology
Volume23
Issue number8
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Glypican-3
  • Hepatoblastoma
  • Insulin-like growth factor 2
  • Wilms tumor

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Hematology
  • Oncology

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