Glycosyltransferase activities in Golgi complex and endoplasmic reticulum fractions isolated from African trypanosomes.

D. J. Grab, S. Ito, U. A. Kara, L. Rovis

Research output: Contribution to journalArticle

Abstract

Highly enriched Golgi complex and endoplasmic reticulum fractions were isolated from total microsomes obtained from Trypanosoma brucei, Trypanosoma congolense, and Trypanosoma vivax, and tested for glycosyltransferase activity. Purity of the fractions was assessed by electron microscopy as well as by biochemical analysis. The relative distribution of all the glycosyltransferases was remarkably similar for the three species of African trypanosomes studied. The Golgi complex fraction contained most of the galactosyltransferase activity followed by the smooth and rough endoplasmic reticulum fractions. The dolichol-dependent mannosyltransferase activities were highest for the rough endoplasmic reticulum, lower for the smooth endoplasmic reticulum, and lowest for the Golgi complex. Although the dolichol-independent form of N-acetylglucosaminyltransferase was essentially similar in all the fractions, the dolichol-dependent form of this enzyme was much higher in the endoplasmic reticulum fractions than in the Golgi complex fraction. Inhibition of this latter activity in the smooth endoplasmic reticulum fraction by tunicamycin A1 suggests that core glycosylation of the variable surface glycoprotein may occur in this organelle and not in the rough endoplasmic reticulum as previously assumed.

Original languageEnglish (US)
Pages (from-to)569-577
Number of pages9
JournalJournal of Cell Biology
Volume99
Issue number2
StatePublished - Aug 1984
Externally publishedYes

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Glycosyltransferases
Trypanosomiasis
Golgi Apparatus
Dolichol
Smooth Endoplasmic Reticulum
Endoplasmic Reticulum
Rough Endoplasmic Reticulum
Trypanosoma vivax
Mannosyltransferases
Trypanosoma congolense
Galactosyltransferases
Trypanosoma brucei brucei
Membrane Glycoproteins
Microsomes
Glycosylation
Organelles
Electron Microscopy
Enzymes

ASJC Scopus subject areas

  • Cell Biology

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Glycosyltransferase activities in Golgi complex and endoplasmic reticulum fractions isolated from African trypanosomes. / Grab, D. J.; Ito, S.; Kara, U. A.; Rovis, L.

In: Journal of Cell Biology, Vol. 99, No. 2, 08.1984, p. 569-577.

Research output: Contribution to journalArticle

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