Glycosphingolipid glycosyl hydrolases and glycosidases of synchronized human KB cells

Subroto B Chatterjee, L. F. Velicer, C. C. Sweeley

Research output: Contribution to journalArticle

Abstract

KB cells were synchronized by a double thymidine block procedure. An investigation was made of the activities of αL fucosidase (EC 3.2.1.51), αD galactosidase (EC 3.2.1.22), βD galactosidase (EC 3.2.1.23), αD glucosidase (EC 3.2.1.20), βD glucosidase (EC 3.2.1.21), αD mannosidase (EC 3.2.1.24), βD N acetylgalactosaminidase (EC 3.2.1.53), BD D N acetylglucosaminidase (EC 3.2.1.52) from synchronized cultures, using appropriate artificial substrates. Ceramide glucosidase (EC 3.2.1.45) and ceramide trihexosidase levels (EC 3.2.1.47) were also investigated at various stages in the cell cycle, using appropriate glycosphingolipid substrates. Whereas each of these enzymes exhibited some activity throughout the cell cycle, peak activity (2 to 6 fold increase) occurred late in the S phase. Two molecular forms of ceramide glucosidase (optimal activity at pH 4.0 and pH 6.0) and two forms of ceramide trihexosidase (pH 4.0 and pH 7.5) were identified. Peak levels of the forms that preferred the relatively acid pH occurred earlier in the S phase of the cell cycle than those of the forms that were more active at the higher pH. The possibility that the forms with optimal activity at pH 4 are precursors of those with optimal activity at pH 6 to 7.5 is discussed. Precipitation of β galactosidase of synchronized KB cells with specific antibody revealed that changes in the activity of this enzyme during the cell cycle were the result of fluctuations in the amount of the enzyme.

Original languageEnglish (US)
Pages (from-to)4972-4979
Number of pages8
JournalJournal of Biological Chemistry
Volume250
Issue number13
StatePublished - 1975
Externally publishedYes

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Glucosidases
KB Cells
Glycosphingolipids
Glycoside Hydrolases
Galactosidases
Hydrolases
Galactosylgalactosylglucosylceramidase
Cells
Ceramides
Cell Cycle
Enzymes
Mannosidases
alpha-L-Fucosidase
Acetylglucosaminidase
Substrates
S Phase
Thymidine
beta-N-Acetyl-Galactosaminidase
Acids
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glycosphingolipid glycosyl hydrolases and glycosidases of synchronized human KB cells. / Chatterjee, Subroto B; Velicer, L. F.; Sweeley, C. C.

In: Journal of Biological Chemistry, Vol. 250, No. 13, 1975, p. 4972-4979.

Research output: Contribution to journalArticle

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