TY - JOUR
T1 - Glycosidases of normal human donor cornea
AU - Bruner, William E.
AU - Stark, Walter J.
N1 - Funding Information:
ACKNOWLEDGEMENTS Supported in part by National Eye Institute fellowship EY 05402 to W.E.B.
PY - 1981
Y1 - 1981
N2 - Although there have been reports of glycosidases in various ocular tissues, there are very few quantitative data concerning human corneal glycosidases. In this study, the pH optima, apparent Km, and Vmax values were determined for seven glycosidases of human donor corneas obtained from an eye bank. In terms of micromoles of substrate cleaved per milligram of protein per hour, the enzymatic activity of the glycosidases was in the following order of decreasing magnitude: βgalactosaminidase, βglucosaminidase, βgalactosidase, βglucuronidase, αmannosidase, αfucosidase, αglucosidase. The pH optima of the enzymes ranged from 3.4 to 5.7.
AB - Although there have been reports of glycosidases in various ocular tissues, there are very few quantitative data concerning human corneal glycosidases. In this study, the pH optima, apparent Km, and Vmax values were determined for seven glycosidases of human donor corneas obtained from an eye bank. In terms of micromoles of substrate cleaved per milligram of protein per hour, the enzymatic activity of the glycosidases was in the following order of decreasing magnitude: βgalactosaminidase, βglucosaminidase, βgalactosidase, βglucuronidase, αmannosidase, αfucosidase, αglucosidase. The pH optima of the enzymes ranged from 3.4 to 5.7.
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U2 - 10.3109/02713688108998368
DO - 10.3109/02713688108998368
M3 - Article
C2 - 7347636
AN - SCOPUS:0019720114
SN - 0271-3683
VL - 1
SP - 705
EP - 710
JO - Current Eye Research
JF - Current Eye Research
IS - 12
ER -