Glycosidases of normal human donor cornea

William E. Bruner, Walter J. Stark

Research output: Contribution to journalArticlepeer-review

Abstract

Although there have been reports of glycosidases in various ocular tissues, there are very few quantitative data concerning human corneal glycosidases. In this study, the pH optima, apparent Km, and Vmax values were determined for seven glycosidases of human donor corneas obtained from an eye bank. In terms of micromoles of substrate cleaved per milligram of protein per hour, the enzymatic activity of the glycosidases was in the following order of decreasing magnitude: βgalactosaminidase, βglucosaminidase, βgalactosidase, βglucuronidase, αmannosidase, αfucosidase, αglucosidase. The pH optima of the enzymes ranged from 3.4 to 5.7.

Original languageEnglish (US)
Pages (from-to)705-710
Number of pages6
JournalCurrent Eye Research
Volume1
Issue number12
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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