Glycosaminoglycans in follicular fluid from women undergoing in vitro fertilization and their relationship to cumulus expansion, fertilization, and development

M. E. Bellin; R. L. Ax; N. Laufer; B. C. Tarlatzis; A. H. DeCherney; D. Feldberg; F. P. Haseltine

doi:10.1016/s0015-0282(16)49162-4

Glycosaminoglycans in follicular fluid from women undergoing in vitro fertilization and their relationship to cumulus expansion, fertilization, and development

M. E. Bellin, R. L. Ax, N. Laufer, B. C. Tarlatzis, A. H. DeCherney, D. Feldberg, F. P. Haseltine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The glycosaminoglycans (GAGs) chondroitin sulfate (CS) and heparan sulfate (HS) were assayed in fluid from 178 individual follicles obtained from women after human menopausal gonadotropin ovulation induction for subsequent in vitro fertilization. CS and HS concentrations ranged from 0 to 3.9 and 0 to 18.6 mg/ml, respectively. The percentage of oocytes fertilized was significantly higher when CS concentrations ranged from 0.3 to 0.8 mg/ml (P < 0.03). As HS levels increased, maturation scores by visual assessment of the egg-corona-cumulus cell complexes increased (P < 0.05), but the percentage of oocytes fertilized was not affected. Cleavage rates of developing embryos were not related to the concentrations of GAGs.

Original language	English (US)
Pages (from-to)	244-248
Number of pages	5
Journal	Fertility and sterility
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/s0015-0282(16)49162-4
State	Published - 1986
Externally published	Yes

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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@article{b9f455a1f97447a3889e152b1ba80c77,

title = "Glycosaminoglycans in follicular fluid from women undergoing in vitro fertilization and their relationship to cumulus expansion, fertilization, and development",

abstract = "The glycosaminoglycans (GAGs) chondroitin sulfate (CS) and heparan sulfate (HS) were assayed in fluid from 178 individual follicles obtained from women after human menopausal gonadotropin ovulation induction for subsequent in vitro fertilization. CS and HS concentrations ranged from 0 to 3.9 and 0 to 18.6 mg/ml, respectively. The percentage of oocytes fertilized was significantly higher when CS concentrations ranged from 0.3 to 0.8 mg/ml (P < 0.03). As HS levels increased, maturation scores by visual assessment of the egg-corona-cumulus cell complexes increased (P < 0.05), but the percentage of oocytes fertilized was not affected. Cleavage rates of developing embryos were not related to the concentrations of GAGs.",

author = "Bellin, {M. E.} and Ax, {R. L.} and N. Laufer and Tarlatzis, {B. C.} and DeCherney, {A. H.} and D. Feldberg and Haseltine, {F. P.}",

note = "Funding Information: Received January 21, 1985; revised and accepted October 17,1985. *Supported by the University of Wisconsin College of Agricultural and Life Sciences, Hatch grant 2778, and the Graduate School. tDepartment of Dairy Science, University of Wisconsin. :j:Reprint requests: Mary E. Bellin, B.S., Project Specialist, Department of Dairy Science, University of Wisconsin, 266 Animal Sciences Building, 1675 Observatory Drive, Madison, Wisconsin 53706. §Endocrine and Reproductive Biology Sections, Department of Obstetrics and Gynecology, Yale University School of Medicine.",

year = "1986",

doi = "10.1016/s0015-0282(16)49162-4",

language = "English (US)",

volume = "45",

pages = "244--248",

journal = "Fertility and Sterility",

issn = "0015-0282",

publisher = "Elsevier Inc.",

number = "2",

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T1 - Glycosaminoglycans in follicular fluid from women undergoing in vitro fertilization and their relationship to cumulus expansion, fertilization, and development

AU - Bellin, M. E.

AU - Ax, R. L.

AU - Laufer, N.

AU - Tarlatzis, B. C.

AU - DeCherney, A. H.

AU - Feldberg, D.

AU - Haseltine, F. P.

N1 - Funding Information: Received January 21, 1985; revised and accepted October 17,1985. *Supported by the University of Wisconsin College of Agricultural and Life Sciences, Hatch grant 2778, and the Graduate School. tDepartment of Dairy Science, University of Wisconsin. :j:Reprint requests: Mary E. Bellin, B.S., Project Specialist, Department of Dairy Science, University of Wisconsin, 266 Animal Sciences Building, 1675 Observatory Drive, Madison, Wisconsin 53706. §Endocrine and Reproductive Biology Sections, Department of Obstetrics and Gynecology, Yale University School of Medicine.

PY - 1986

Y1 - 1986

N2 - The glycosaminoglycans (GAGs) chondroitin sulfate (CS) and heparan sulfate (HS) were assayed in fluid from 178 individual follicles obtained from women after human menopausal gonadotropin ovulation induction for subsequent in vitro fertilization. CS and HS concentrations ranged from 0 to 3.9 and 0 to 18.6 mg/ml, respectively. The percentage of oocytes fertilized was significantly higher when CS concentrations ranged from 0.3 to 0.8 mg/ml (P < 0.03). As HS levels increased, maturation scores by visual assessment of the egg-corona-cumulus cell complexes increased (P < 0.05), but the percentage of oocytes fertilized was not affected. Cleavage rates of developing embryos were not related to the concentrations of GAGs.

AB - The glycosaminoglycans (GAGs) chondroitin sulfate (CS) and heparan sulfate (HS) were assayed in fluid from 178 individual follicles obtained from women after human menopausal gonadotropin ovulation induction for subsequent in vitro fertilization. CS and HS concentrations ranged from 0 to 3.9 and 0 to 18.6 mg/ml, respectively. The percentage of oocytes fertilized was significantly higher when CS concentrations ranged from 0.3 to 0.8 mg/ml (P < 0.03). As HS levels increased, maturation scores by visual assessment of the egg-corona-cumulus cell complexes increased (P < 0.05), but the percentage of oocytes fertilized was not affected. Cleavage rates of developing embryos were not related to the concentrations of GAGs.

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VL - 45

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JO - Fertility and Sterility

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Glycosaminoglycans in follicular fluid from women undergoing in vitro fertilization and their relationship to cumulus expansion, fertilization, and development

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