Glycosaminoglycan content in neonatal rat aortic smooth muscle cell cultures

H. Ohishi, D. Hess, M. Kosakai, K. Schmid, J. Hausmann, Peter Mogayzel, B. Faris, C. Franzblau

Research output: Contribution to journalArticle

Abstract

In the present study the biosynthesis of glycosaminoglycans (GAGs) by neonatal rat aortic smooth muscle cells in culture was studied. Heparan sulfate (HS) was the predominant GAG of the cell layer accounting for 32-49% of the total GAGs depending on the time in culture. The presence of low sulfated chondroitin sulfate (LSC) in aortic smooth muscle cell cultures is reported here for the first time. The effect of ascorbate on the synthesis and accumulation of these macromolecules resulted in a relative increase of C4S and DS in the cell layer. In contrast, the distribution of the GAGs which were secreted into the medium was not significantly effected by the addition of ascorbate. While HS was always found to be a minor component, the other GAGs were present in about equal concentrations. The total GAG accumulation in the medium was much greater (91-97%) than that of the cell layer (3-9%) indicating that the cells are synthesizing relatively large amounts of GAGs, although incorporation of these macromolecules into the extracellular matrix was consistenly low.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalAtherosclerosis
Volume69
Issue number1
DOIs
Publication statusPublished - 1988
Externally publishedYes

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Keywords

  • Aortic rat smooth muscle cell cultures
  • Ascorbate
  • Glycosaminoglycans

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Ohishi, H., Hess, D., Kosakai, M., Schmid, K., Hausmann, J., Mogayzel, P., ... Franzblau, C. (1988). Glycosaminoglycan content in neonatal rat aortic smooth muscle cell cultures. Atherosclerosis, 69(1), 61-68. https://doi.org/10.1016/0021-9150(88)90289-4