Glycoproteomic analysis of bronchoalveolar lavage (BAL) fluid identifies tumor-associated glycoproteins from lung adenocarcinoma

Qing Kay Li, Punit Shah, Yan Li, Paul O. Aiyetan, Jing Chen, Rex Yung, Daniela Molena, Edward Gabrielson, Frederic Askin, Daniel W. Chan, Hui Zhang

Research output: Contribution to journalArticle

Abstract

Cytological examination of cells from bronchoalveolar lavage (BAL) is commonly used for the diagnosis of lung cancer. Proteins released from lung cancer cells into BAL may serve as biomarkers for cancer detection. In this study, N-glycoproteins in eight cases of BAL fluid, as well as eight lung adenocarcinoma tissues and eight tumor-matched normal lung tissues, were analyzed using the solid-phase extraction of N-glycoprotein (SPEG), iTRAQ labeling, and liquid chromatography tandem mass spectrometry (LC-MS/MS). Of 80 glycoproteins found in BAL specimens, 32 were identified in both cancer BAL and cancer tissues, with levels of 25 glycoproteins showing at least a 2-fold difference between cancer and benign BAL. Among them, eight glycoproteins showed greater than 2-fold elevations in cancer BAL, including Neutrophil elastase (NE), Integrin alpha-M, Cullin-4B, Napsin A, lysosome-associated membrane protein 2 (LAMP2), Cathepsin D, BPI fold-containing family B member 2, and Neutrophil gelatinase-associated lipocalin. The levels of Napsin A in cancer BAL were further verified in independently collected 39 BAL specimens using an ELISA assay. Our study demonstrates that potential protein biomarkers in BAL fluid can be detected and quantified.

Original languageEnglish (US)
Pages (from-to)3689-3696
Number of pages8
JournalJournal of proteome research
Volume12
Issue number8
DOIs
StatePublished - Aug 2 2013

Keywords

  • bronchoalveolar lavage (BAL)
  • glycoproteins
  • glycoproteomics
  • lung adenocarcinoma
  • nonsmall cell lung cancer (NSCLC)

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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