Glycoprotein synthesis in colonic epithelial cells from rats

Anuradha Mukherjee, Rabi Patnaik, Belur S. Bhagavan, P. P. Nair

Research output: Contribution to journalArticlepeer-review

Abstract

Viable colonic epithelial cells from rats were isolated by a non-enzymatic procedure using EDTA. The isolated cells were fractionated by sedimentation through a 15% to 35% discontinuous Ficoll gradient to yield cells differing in proliferative capacity from three different density regions of the gradient. Glycoprotein synthesis of these fractionated cells was examined in terms of their ability to absorb and incorporate labeled glucosamine and fucose into trichloroacetic acid precipitable material. Glycoprotein synthesis was highest among cells with intermediate densities, banding in the middle of the gradient (Fraction II). Cytomorphological examination showed that these cells were predominantly goblet type.

Original languageEnglish (US)
Pages (from-to)409-415
Number of pages7
JournalCell Biology International Reports
Volume5
Issue number4
DOIs
StatePublished - Apr 1981

ASJC Scopus subject areas

  • Cell Biology

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