Glycomic analysis of human mast cells, eosinophils and basophils

Simon J. North, Stephan Von Gunten, Aristotelis Antonopoulos, Alana Trollope, Donald Macglashan, Jihye Jang-Lee, Anne Dell, Dean D. Metcalfe, Arnold S. Kirshenbaum, Bruce S. Bochner, Stuart M. Haslam

Research output: Contribution to journalArticle

Abstract

In allergic diseases such as asthma, eosinophils, basophils and mast cells, through release of preformed and newly generated mediators, granule proteins and cytokines, are recognized as key effector cells. While their surface protein phenotypes, mediator release profiles, ontogeny, cell trafficking and genomes have been generally explored and compared, there has yet to be any thorough analysis and comparison of their glycomes. Such studies are critical to understand the contribution of carbohydrates to the induction and regulation of allergic inflammatory responses and are now possible using improved technologies for detecting and characterizing cell-derived glycans. We thus report here the application of high-sensitivity mass spectrometric-based glycomics methodologies to the analysis of N-linked glycans derived from isolated populations of human mast cells, eosinophils and basophils. The samples were subjected to matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) screening analyses and MALDI-TOF/TOF sequencing studies. Results reveal substantive quantities of terminal N-acetylglucosamine containing structures in both the eosinophil and the basophil samples, whereas mast cells display greater relative quantities of sialylated terminal epitopes. For the first time, we characterize the cell surface glycan structures of principal allergic effector cells, which by interaction with glycan-binding proteins (e.g. lectins) have the possibility to dictate cellular functions, and might thus have important implications for the pathogenesis of inflammatory and allergic diseases.

Original languageEnglish (US)
Pages (from-to)12-22
Number of pages11
JournalGlycobiology
Volume22
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Glycomics
Basophils
Eosinophils
Mast Cells
Polysaccharides
Ionization
Desorption
Lasers
Acetylglucosamine
Lectins
Cell Communication
Epitopes
Carrier Proteins
Screening
Membrane Proteins
Asthma
Genes
Carbohydrates
Genome
Cytokines

Keywords

  • basophil
  • eosinophil
  • glycomics
  • mass spectrometry
  • mast cell

ASJC Scopus subject areas

  • Biochemistry

Cite this

North, S. J., Von Gunten, S., Antonopoulos, A., Trollope, A., Macglashan, D., Jang-Lee, J., ... Haslam, S. M. (2012). Glycomic analysis of human mast cells, eosinophils and basophils. Glycobiology, 22(1), 12-22. https://doi.org/10.1093/glycob/cwr089

Glycomic analysis of human mast cells, eosinophils and basophils. / North, Simon J.; Von Gunten, Stephan; Antonopoulos, Aristotelis; Trollope, Alana; Macglashan, Donald; Jang-Lee, Jihye; Dell, Anne; Metcalfe, Dean D.; Kirshenbaum, Arnold S.; Bochner, Bruce S.; Haslam, Stuart M.

In: Glycobiology, Vol. 22, No. 1, 01.2012, p. 12-22.

Research output: Contribution to journalArticle

North, SJ, Von Gunten, S, Antonopoulos, A, Trollope, A, Macglashan, D, Jang-Lee, J, Dell, A, Metcalfe, DD, Kirshenbaum, AS, Bochner, BS & Haslam, SM 2012, 'Glycomic analysis of human mast cells, eosinophils and basophils', Glycobiology, vol. 22, no. 1, pp. 12-22. https://doi.org/10.1093/glycob/cwr089
North SJ, Von Gunten S, Antonopoulos A, Trollope A, Macglashan D, Jang-Lee J et al. Glycomic analysis of human mast cells, eosinophils and basophils. Glycobiology. 2012 Jan;22(1):12-22. https://doi.org/10.1093/glycob/cwr089
North, Simon J. ; Von Gunten, Stephan ; Antonopoulos, Aristotelis ; Trollope, Alana ; Macglashan, Donald ; Jang-Lee, Jihye ; Dell, Anne ; Metcalfe, Dean D. ; Kirshenbaum, Arnold S. ; Bochner, Bruce S. ; Haslam, Stuart M. / Glycomic analysis of human mast cells, eosinophils and basophils. In: Glycobiology. 2012 ; Vol. 22, No. 1. pp. 12-22.
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