This study confirms the feasibility of using nuclear magnetic resonance (NMR) spectroscopy to assess, noninvasively, glucose metabolism in isolated corneal tissue. Glycolytic activity of human corneas was analyzed by high-resolution NMR spectroscopy and spectrophotometric coupling assays. Glucose utilization and lactate formation were readily demonstrated in incubating intact and de-epithelialized human corneas utilizing an NMR spectrometer with an array of spectra taken at ten-minute intervals over a period of nine hours. The lactate formation rate by biochemical coupling assay was determined to be approximately 0.50 μmol/hr in the intact cornea and 0.20 μmol/hr in the de-epithelialized cornea. Analysis by NMR spectroscopy of the rate of glucose utilization and lactate formation may be a useful means of determining human donor corneal viability.
|Original language||English (US)|
|Number of pages||4|
|Journal||Archives of ophthalmology|
|State||Published - Jan 1 1986|
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