Glycolipids support E-selectin-specific strong cell tethering under flow

Monica M. Burdick, Bruce S. Bochner, Brian E. Collins, Ronald L. Schnaar, Konstantinos Konstantopoulos

Research output: Contribution to journalArticlepeer-review

Abstract

This study provides functional evidence that glycosphingolipids constitute ligands for E-selectin but not P-selectin. Chinese hamster ovary (CHO) cells expressing E-selectin (CHO-E) or P-selectin (CHO-P) were perfused over α2,3-sialyl Lewis X (α2,3-sLex) presented as the hexaosylceramide glycosphingolipid adsorbed in a monolayer containing phosphatidylcholine and cholesterol. CHO-E cells tethered extensively and formed slow, stable rolling interactions with α2,3-sLex glycosphingolipid but not with the comparable α2,6-sLex glycosphingolipid. Tethering/rolling varied with wall shear stress, selectin density, and ligand density. In contrast, α2,3-sLex glycosphingolipid supported only limited, fast CHO-P cell rolling. As calculated from a stochastic model of cell rolling, the step size between successive bond releases from the α2,3-sLex glycosphingolipid was smaller for CHO-E than CHO-P cells, whereas the opposite effect was observed for the waiting time between these events. Detachment assays revealed stronger adhesive interactions of CHO-E than CHO-P cells with α2,3-sLex glycosphingolipid. These findings indicate that glycosphingolipids expressing an appropriate oligosaccharide mediate cell tethering/rolling via E-selectin but not P-selectin.

Original languageEnglish (US)
Pages (from-to)42-49
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume284
Issue number1
DOIs
StatePublished - 2001

Keywords

  • Selectins
  • Shear
  • Sialyl Lewis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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