Glycolipid antigen expression in human lung cancer

S. L. Spitalnik, P. F. Spitalnik, C. Dubois, J. Mulshine, J. L. Magnani, F. Cuttitta, C. I. Civin, J. D. Minna, V. Ginsburg

Research output: Contribution to journalArticle

Abstract

Several mouse monoclonal antibodies which recognize carbohydrate sequences distinguish between different types of human lung cancer immunohistologically. These antibodies bind to glycolipid antigens produced by the cancer cells. When these glycolipids are separated by thin-layer chromatography, immunostaining of the chromatograms yields complex patterns of antigen-positive bands. To determine whether glycolipid patterns are useful in the classification of lung cancer, 16 human lung cancer cell lines comprising the major histological types of primary lung cancer were studied. Neutral glycolipids and gangliosides were isolated and separated by thin-layer chromatography. Six anti-carbohydrate antibodies which recognize structurally related antigens were used for immunostaining. Neuraminidase treatment of the chromatograms was used to detect 'cryptic' sialylated antigens. All the cell lines were unique with regard to the type, amount, and chromatography pattern of the glycolipid antigens produced. Small cell lung cancer cell lines synthesized the greatest variety of antigens, whereas cell lines with large cell cytology synthesized the least. Interestingly, there was an inverse relationship between expression of some glycolipid antigens and DNA amplification of the c-myc oncogene. This suggests that enhanced c-myc expression may influence the types of glycolipids expressed at the surface of lung tumor cells.

Original languageEnglish (US)
Pages (from-to)4751-4755
Number of pages5
JournalCancer Research
Volume46
Issue number9
StatePublished - 1986
Externally publishedYes

Fingerprint

Glycolipids
Lung Neoplasms
Antigens
Cell Line
Thin Layer Chromatography
Carbohydrate Sequence
myc Genes
Gangliosides
Small Cell Lung Carcinoma
Neuraminidase
Cell Biology
Chromatography
Anti-Idiotypic Antibodies
Neoplasms
Monoclonal Antibodies
Carbohydrates
Lung
Antibodies
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Spitalnik, S. L., Spitalnik, P. F., Dubois, C., Mulshine, J., Magnani, J. L., Cuttitta, F., ... Ginsburg, V. (1986). Glycolipid antigen expression in human lung cancer. Cancer Research, 46(9), 4751-4755.

Glycolipid antigen expression in human lung cancer. / Spitalnik, S. L.; Spitalnik, P. F.; Dubois, C.; Mulshine, J.; Magnani, J. L.; Cuttitta, F.; Civin, C. I.; Minna, J. D.; Ginsburg, V.

In: Cancer Research, Vol. 46, No. 9, 1986, p. 4751-4755.

Research output: Contribution to journalArticle

Spitalnik, SL, Spitalnik, PF, Dubois, C, Mulshine, J, Magnani, JL, Cuttitta, F, Civin, CI, Minna, JD & Ginsburg, V 1986, 'Glycolipid antigen expression in human lung cancer', Cancer Research, vol. 46, no. 9, pp. 4751-4755.
Spitalnik SL, Spitalnik PF, Dubois C, Mulshine J, Magnani JL, Cuttitta F et al. Glycolipid antigen expression in human lung cancer. Cancer Research. 1986;46(9):4751-4755.
Spitalnik, S. L. ; Spitalnik, P. F. ; Dubois, C. ; Mulshine, J. ; Magnani, J. L. ; Cuttitta, F. ; Civin, C. I. ; Minna, J. D. ; Ginsburg, V. / Glycolipid antigen expression in human lung cancer. In: Cancer Research. 1986 ; Vol. 46, No. 9. pp. 4751-4755.
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