Glycinergic feedback enhances synaptic gain in the distal retina

Zheng Jiang, Jinnan Yang, Lauren A. Purpura, Yufei Liu, Harris Ripps, Wen Shen

Research output: Contribution to journalArticle

Abstract

Glycine input originates with interplexiform cells, a group of neurons situated within the inner retina that transmit signals centrifugally to the distal retina. The effect on visual function of this novel mechanism is largely unknown. Using gramicidin-perforated patch whole cell recordings, intracellular recordings and specific antibody labelling techniques, we examined the effects of the synaptic connections between glycinergic interplexiform cells, photoreceptors and bipolar cells. To confirm that interplexiform cells make centrifugal feedback on bipolar cell dendrites, we recorded the postsynaptic glycine currents from axon-detached bipolar cells while stimulating presynaptic interplexiform cells. The results show that glycinergic interplexiform cells activate bipolar cell dendrites that express the α3 subunit of the glycine receptor, as well as a subclass of unidentified receptors on photoreceptors. By virtue of their synaptic contacts, glycine centrifugal feedback increases glutamate release from photoreceptors and suppresses the uptake of glutamate by the type 2A excitatory amino acid transporter on photoreceptors. The net effect is a significant increase in synaptic gain between photoreceptors and their second-order neurons.

Original languageEnglish (US)
Pages (from-to)1479-1492
Number of pages14
JournalJournal of Physiology
Volume592
Issue number7
DOIs
StatePublished - Apr 1 2014
Externally publishedYes

Fingerprint

Retina
Glycine
Dendrites
Glutamic Acid
Glycine Receptors
Amino Acid Transport Systems
Gramicidin
Neurons
Synaptic Potentials
Photoreceptor Cells
Excitatory Amino Acids
Patch-Clamp Techniques
Axons
Antibodies

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)

Cite this

Jiang, Z., Yang, J., Purpura, L. A., Liu, Y., Ripps, H., & Shen, W. (2014). Glycinergic feedback enhances synaptic gain in the distal retina. Journal of Physiology, 592(7), 1479-1492. https://doi.org/10.1113/jphysiol.2013.265785

Glycinergic feedback enhances synaptic gain in the distal retina. / Jiang, Zheng; Yang, Jinnan; Purpura, Lauren A.; Liu, Yufei; Ripps, Harris; Shen, Wen.

In: Journal of Physiology, Vol. 592, No. 7, 01.04.2014, p. 1479-1492.

Research output: Contribution to journalArticle

Jiang, Z, Yang, J, Purpura, LA, Liu, Y, Ripps, H & Shen, W 2014, 'Glycinergic feedback enhances synaptic gain in the distal retina', Journal of Physiology, vol. 592, no. 7, pp. 1479-1492. https://doi.org/10.1113/jphysiol.2013.265785
Jiang Z, Yang J, Purpura LA, Liu Y, Ripps H, Shen W. Glycinergic feedback enhances synaptic gain in the distal retina. Journal of Physiology. 2014 Apr 1;592(7):1479-1492. https://doi.org/10.1113/jphysiol.2013.265785
Jiang, Zheng ; Yang, Jinnan ; Purpura, Lauren A. ; Liu, Yufei ; Ripps, Harris ; Shen, Wen. / Glycinergic feedback enhances synaptic gain in the distal retina. In: Journal of Physiology. 2014 ; Vol. 592, No. 7. pp. 1479-1492.
@article{ecf1adc6691548319ee9b71c0d2d4c44,
title = "Glycinergic feedback enhances synaptic gain in the distal retina",
abstract = "Glycine input originates with interplexiform cells, a group of neurons situated within the inner retina that transmit signals centrifugally to the distal retina. The effect on visual function of this novel mechanism is largely unknown. Using gramicidin-perforated patch whole cell recordings, intracellular recordings and specific antibody labelling techniques, we examined the effects of the synaptic connections between glycinergic interplexiform cells, photoreceptors and bipolar cells. To confirm that interplexiform cells make centrifugal feedback on bipolar cell dendrites, we recorded the postsynaptic glycine currents from axon-detached bipolar cells while stimulating presynaptic interplexiform cells. The results show that glycinergic interplexiform cells activate bipolar cell dendrites that express the α3 subunit of the glycine receptor, as well as a subclass of unidentified receptors on photoreceptors. By virtue of their synaptic contacts, glycine centrifugal feedback increases glutamate release from photoreceptors and suppresses the uptake of glutamate by the type 2A excitatory amino acid transporter on photoreceptors. The net effect is a significant increase in synaptic gain between photoreceptors and their second-order neurons.",
author = "Zheng Jiang and Jinnan Yang and Purpura, {Lauren A.} and Yufei Liu and Harris Ripps and Wen Shen",
year = "2014",
month = "4",
day = "1",
doi = "10.1113/jphysiol.2013.265785",
language = "English (US)",
volume = "592",
pages = "1479--1492",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Glycinergic feedback enhances synaptic gain in the distal retina

AU - Jiang, Zheng

AU - Yang, Jinnan

AU - Purpura, Lauren A.

AU - Liu, Yufei

AU - Ripps, Harris

AU - Shen, Wen

PY - 2014/4/1

Y1 - 2014/4/1

N2 - Glycine input originates with interplexiform cells, a group of neurons situated within the inner retina that transmit signals centrifugally to the distal retina. The effect on visual function of this novel mechanism is largely unknown. Using gramicidin-perforated patch whole cell recordings, intracellular recordings and specific antibody labelling techniques, we examined the effects of the synaptic connections between glycinergic interplexiform cells, photoreceptors and bipolar cells. To confirm that interplexiform cells make centrifugal feedback on bipolar cell dendrites, we recorded the postsynaptic glycine currents from axon-detached bipolar cells while stimulating presynaptic interplexiform cells. The results show that glycinergic interplexiform cells activate bipolar cell dendrites that express the α3 subunit of the glycine receptor, as well as a subclass of unidentified receptors on photoreceptors. By virtue of their synaptic contacts, glycine centrifugal feedback increases glutamate release from photoreceptors and suppresses the uptake of glutamate by the type 2A excitatory amino acid transporter on photoreceptors. The net effect is a significant increase in synaptic gain between photoreceptors and their second-order neurons.

AB - Glycine input originates with interplexiform cells, a group of neurons situated within the inner retina that transmit signals centrifugally to the distal retina. The effect on visual function of this novel mechanism is largely unknown. Using gramicidin-perforated patch whole cell recordings, intracellular recordings and specific antibody labelling techniques, we examined the effects of the synaptic connections between glycinergic interplexiform cells, photoreceptors and bipolar cells. To confirm that interplexiform cells make centrifugal feedback on bipolar cell dendrites, we recorded the postsynaptic glycine currents from axon-detached bipolar cells while stimulating presynaptic interplexiform cells. The results show that glycinergic interplexiform cells activate bipolar cell dendrites that express the α3 subunit of the glycine receptor, as well as a subclass of unidentified receptors on photoreceptors. By virtue of their synaptic contacts, glycine centrifugal feedback increases glutamate release from photoreceptors and suppresses the uptake of glutamate by the type 2A excitatory amino acid transporter on photoreceptors. The net effect is a significant increase in synaptic gain between photoreceptors and their second-order neurons.

UR - http://www.scopus.com/inward/record.url?scp=84897437424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897437424&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2013.265785

DO - 10.1113/jphysiol.2013.265785

M3 - Article

VL - 592

SP - 1479

EP - 1492

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 7

ER -