Glycine reverses 7-chlorokynurenic acid-induced inhibition of [3H]MK-801 binding

Ratna Sircar, Mark J. Frusciante, Daniel C. Javiet, Stephen R. Zukin

Research output: Contribution to journalArticle

Abstract

7-Chlorokynurenic acid (7-Cl KYNA) has been reported to attenuate N-methyl-d-aspartate (NMDA) receptor functioning by a potent and selective inhibitory action mediated at the strychnine-insensitive glycine recognition site of the NMDA complex. Here we report that 7-Cl KYNA dose-dependently inhibits [3H]MK-801 binding to the PCP receptor, and that this effect is reversed by addittion of glycine. Since [3H]MK-801 binding is a measure of channel activation, our results are consistents with the hypothesis that 7-Cl KYNA exerts its NMDA receptor antagonism by acting at the glycine site, and that activation of the glycine site is required for NMDA channel activity to occur.

Original languageEnglish (US)
Pages (from-to)325-327
Number of pages3
JournalBrain Research
Volume504
Issue number2
DOIs
Publication statusPublished - Dec 18 1989
Externally publishedYes

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Keywords

  • 7-Chlorokynurenic acid
  • Glycine receptor antagonist
  • N-methyl-d-aspartate receptor
  • Phencyclidine receptor
  • [H]MK-801 binding

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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