Glycerol-3-phosphate acyltransferase contributes to triacylglycerol biosynthesis, lipid droplet formation, and host invasion in Metarhizium robertsii

Qiang Gao, Yanfang Shang, Wei Huang, Chengshu Wang

Research output: Contribution to journalArticle

Abstract

Enzymes involved in the triacylglycerol (TAG) biosynthesis have been well studied in the model organisms of yeasts and animals. Among these, the isoforms of glycerol-3-phosphate acyltransferase (GPAT) redundantly catalyze the first and rate-limiting step in glycerolipid synthesis. Here, we report the functions of mrGAT, a GPAT ortholog, in an insect-pathogenic fungus, Metarhizium robertsii. Unlike in yeasts and animals, a single copy of the mrGAT gene is present in the fungal genome and the gene deletion mutant is viable. Compared to the wild type and the gene-rescued mutant, the ΔmrGAT mutant demonstrated reduced abilities to produce conidia and synthesize TAG, glycerol, and total lipids. More importantly, we found that mrGAT is localized to the endoplasmic reticulum and directly linked to the formation of lipid droplets (LDs) in fungal cells. Insect bioassay results showed that mrGAT is required for full fungal virulence by aiding fungal penetration of host cuticles. Data from this study not only advance our understanding of GPAT functions in fungi but also suggest that filamentous fungi such as M. robertsii can serve as a good model to elucidate the role of the glycerol phosphate pathway in fungal physiology, particularly to determine the mechanistic connection of GPAT to LD formation.

Original languageEnglish (US)
Pages (from-to)7646-7653
Number of pages8
JournalApplied and Environmental Microbiology
Volume79
Issue number24
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

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Metarhizium
glycerol-3-phosphate acyltransferase
Acyltransferases
droplets
droplet
Triglycerides
lipid
triacylglycerols
phosphate
biosynthesis
Fungi
lipids
Glycerol
mutants
fungi
Insects
fungus
glycerol
fungus physiology
Fungal Genome

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Glycerol-3-phosphate acyltransferase contributes to triacylglycerol biosynthesis, lipid droplet formation, and host invasion in Metarhizium robertsii. / Gao, Qiang; Shang, Yanfang; Huang, Wei; Wang, Chengshu.

In: Applied and Environmental Microbiology, Vol. 79, No. 24, 01.12.2013, p. 7646-7653.

Research output: Contribution to journalArticle

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