TY - JOUR
T1 - Glyceraldehyde 3-phosphate dehydrogenase is an enzyme-crystallin in diurnal geckos of the genus Phelsuma
AU - Jimenez-Asensio, Jose
AU - Gonzalez, Pedro
AU - Samuel Zigler, J.
AU - Garland, Donita L.
PY - 1995/4/26
Y1 - 1995/4/26
N2 - Lenses from diurnal geckos, Phelsuma barbouri, Phelsuma madagascariensis grandis and Phelsuma serraticauda contain a prominent 37 kDa polypeptide (π-crystallin) that is not present in lenses from the nocturnal geckos, Gekko gekko, Hemidactylus garnoti, Tarentola annularis, and Uroplatus henkeli. This protein was partially purified from P. serraticauda and was identified as glyceraldehyde 3-phosphate dehydrogenase (GAPD). The GAPDs, which constitute 14-24% of the lens soluble protein in the diurnal species, were highly active. The presence of this enzyme in the lenses of diurnal, but not nocturnal, geckos supports the hypothesis that the oxido-reductases found as enzyme-crystallins may function in providing protection against the increased oxidative stress to which diurnal species are exposed. These findings strongly support the concept that the recruitment of enzyme-crystallins is a selective response to changes in the visual environment.
AB - Lenses from diurnal geckos, Phelsuma barbouri, Phelsuma madagascariensis grandis and Phelsuma serraticauda contain a prominent 37 kDa polypeptide (π-crystallin) that is not present in lenses from the nocturnal geckos, Gekko gekko, Hemidactylus garnoti, Tarentola annularis, and Uroplatus henkeli. This protein was partially purified from P. serraticauda and was identified as glyceraldehyde 3-phosphate dehydrogenase (GAPD). The GAPDs, which constitute 14-24% of the lens soluble protein in the diurnal species, were highly active. The presence of this enzyme in the lenses of diurnal, but not nocturnal, geckos supports the hypothesis that the oxido-reductases found as enzyme-crystallins may function in providing protection against the increased oxidative stress to which diurnal species are exposed. These findings strongly support the concept that the recruitment of enzyme-crystallins is a selective response to changes in the visual environment.
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U2 - 10.1006/bbrc.1995.1570
DO - 10.1006/bbrc.1995.1570
M3 - Article
C2 - 7733971
AN - SCOPUS:0029068413
SN - 0006-291X
VL - 209
SP - 796
EP - 802
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -