Glycan microarray analysis of Candida glabrata adhesin ligand specificity

Margaret L. Zupancic, Matthew Frieman, David Smith, Richard A. Alvarez, Richard D. Cummings, Brendan P Cormack

Research output: Contribution to journalArticle

Abstract

The Candida glabrata genome encodes at least 23 members of the EPA (epithelial adhesin) family responsible for mediating adherence to host cells. To better understand the mechanism by which the Epa proteins contribute to pathogenesis, we have used glycan microarray analysis to characterize their carbohydrate-binding specificities. Using Saccharomyces cerevisiae strains surface-expressing the N-terminal ligand-binding domain of the Epa proteins, we found that the three Epa family members functionally identified as adhesins in Candida glabrata (Epa1, Epa6 and Epa7) bind to ligands containing a terminal galactose residue. However, the specificity of the three proteins for glycans within this class varies, with Epa6 having a broader specificity range than Epa1 or Epa7. This result is intriguing given the close homology between Epa6 and Epa7, which are 92% identical at the amino acid level. We have mapped a five-amino-acid region within the N-terminal ligand-binding domain that accounts for the difference in specificity of Epa6 and Epa7 and show that these residues contribute to adherence to both epithelial and endothelial cell lines in vitro.

Original languageEnglish (US)
Pages (from-to)547-559
Number of pages13
JournalMolecular Microbiology
Volume68
Issue number3
DOIs
StatePublished - May 2008

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Candida glabrata
Microarray Analysis
Polysaccharides
Ligands
Amino Acids
Galactose
Saccharomyces cerevisiae
Proteins
Endothelial Cells
Epithelial Cells
Carbohydrates
Genome
Cell Line

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Glycan microarray analysis of Candida glabrata adhesin ligand specificity. / Zupancic, Margaret L.; Frieman, Matthew; Smith, David; Alvarez, Richard A.; Cummings, Richard D.; Cormack, Brendan P.

In: Molecular Microbiology, Vol. 68, No. 3, 05.2008, p. 547-559.

Research output: Contribution to journalArticle

Zupancic, Margaret L. ; Frieman, Matthew ; Smith, David ; Alvarez, Richard A. ; Cummings, Richard D. ; Cormack, Brendan P. / Glycan microarray analysis of Candida glabrata adhesin ligand specificity. In: Molecular Microbiology. 2008 ; Vol. 68, No. 3. pp. 547-559.
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