Glycan analysis by reversible reaction to hydrazide beads and mass spectrometry

Shuang J. Yang, Hui Zhang

Research output: Contribution to journalArticle

Abstract

Investigation into glycoproteins and their associated glycans is the key to understanding the function of glycoproteins in biological pathways and disease development. Current methods for glycan analysis are generally based on multiple preparation processes to separate glycans from proteins and other molecules prior to analysis. During the multistep purification processes, glycans are continuously lost and the procedure increases the difficulty for accurate quantitative analysis of glycans. Here we describe the development of a novel technique, which uses hydrazide beads to capture glycans. It is based on the conjugation of glycans to hydrazide beads through the formation of reversible hydrazone, washing out unbound nonglycans, then releasing captured glycans by acids. The results showed that the glycans were able to be isolated from concatenate peptides by using hydrazide beads. This technique was also applied to the analysis of glycans from sera sample. The integrated capture-release on the solid-phase simplifies the procedure for glycan preparation from a complex mixture and can be a powerful tool for glycan analysis.

Original languageEnglish (US)
Pages (from-to)2232-2238
Number of pages7
JournalAnalytical Chemistry
Volume84
Issue number5
DOIs
StatePublished - Mar 6 2012

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Mass spectrometry
Polysaccharides
Glycoproteins
Hydrazones
Complex Mixtures
Washing
Purification
Peptides
Molecules
Acids

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Glycan analysis by reversible reaction to hydrazide beads and mass spectrometry. / Yang, Shuang J.; Zhang, Hui.

In: Analytical Chemistry, Vol. 84, No. 5, 06.03.2012, p. 2232-2238.

Research output: Contribution to journalArticle

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