Glutathione S-transferase iso-enzymes in perfusate from pumped kidneys are associated with delayed graft function

I. E. Hall, R. S. Bhangoo, P. P. Reese, M. D. Doshi, F. L. Weng, K. Hong, H. Lin, G. Han, R. D. Hasz, M. J. Goldstein, B. Schröppel, C. R. Parikh

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Accurate and reliable assessment tools are needed in transplantation. The objective of this prospective, multi-center study was to determine the associations of the alpha and pi iso-enzymes of glutathione S-transferase (GST), measured from perfusate solution at the start and end (base and post) of kidney allograft machine perfusion, with subsequent delayed graft function (DGF). We also compared GST iso-enzyme perfusate levels from discarded versus transplanted kidneys. A total of 428 kidneys were linked to outcomes as recorded by the United Network of Organ Sharing. DGF, defined as any dialysis in the first week of transplant, occurred in 141 recipients (32%). Alpha- and pi-GST levels significantly increased during machine perfusion. The adjusted relative risks (95% confidence interval) of DGF with each log-unit increase in base and post pi-GST were 1.14 (1.0-1.3) and 1.36 (1.1-1.8), respectively. Alpha-GST was not independently associated with DGF. There were no significant differences in GST values between discarded and transplanted kidneys, though renal resistance was significantly higher in discarded kidneys. We found pi-GST at the end of machine perfusion to be independently associated with DGF. Further studies should elucidate the utility of GST for identifying injured kidneys with regard to organ allocation, discard and recipient management decisions.

Original languageEnglish (US)
Pages (from-to)886-896
Number of pages11
JournalAmerican Journal of Transplantation
Issue number4
StatePublished - Apr 2014
Externally publishedYes


  • Biomarker
  • ischemia
  • kidney injury
  • perfusion pumping

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)


Dive into the research topics of 'Glutathione S-transferase iso-enzymes in perfusate from pumped kidneys are associated with delayed graft function'. Together they form a unique fingerprint.

Cite this