Glutathione-dependent dechlorination of chloramphenicol by cytosol of rat liver

J. L. Martin; J. W. George; L. R. Pohl

Glutathione-dependent dechlorination of chloramphenicol by cytosol of rat liver

J. L. Martin, J. W. George, L. R. Pohl

School of Medicine

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The 100,000 g supernatant fraction (cytosol) of rat liver converts chloramphenicol (CAP, RNHCOCHCl₂) into two products. Most of the enzyme activity is lost during dialysis of the enzyme preparation overnight, but is restored by addition of glutathione (GSH). Other thiols are not as effective as GSH in restoring the enzyme activity. The formation of the metabolites is not inhibited when incubations are performed under anaerobic conditions. The major metabolite was identified as CAP aldehyde (RNHCOCHO) whereas the minor metabolite was identified as an alkali-unstable derivative of CAP oxamic acid (RNHCOCOOH). Plausible pathways are discussed for the formation of these metabolites.

Original language	English (US)
Pages (from-to)	93-97
Number of pages	5
Journal	Drug Metabolism and Disposition
Volume	8
Issue number	2
State	Published - Jan 1 1980

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

Cite this

@article{8b35b9bd6ff44221b8baa63b7041c8d9,

title = "Glutathione-dependent dechlorination of chloramphenicol by cytosol of rat liver",

abstract = "The 100,000 g supernatant fraction (cytosol) of rat liver converts chloramphenicol (CAP, RNHCOCHCl2) into two products. Most of the enzyme activity is lost during dialysis of the enzyme preparation overnight, but is restored by addition of glutathione (GSH). Other thiols are not as effective as GSH in restoring the enzyme activity. The formation of the metabolites is not inhibited when incubations are performed under anaerobic conditions. The major metabolite was identified as CAP aldehyde (RNHCOCHO) whereas the minor metabolite was identified as an alkali-unstable derivative of CAP oxamic acid (RNHCOCOOH). Plausible pathways are discussed for the formation of these metabolites.",

author = "Martin, {J. L.} and George, {J. W.} and Pohl, {L. R.}",

year = "1980",

month = jan,

day = "1",

language = "English (US)",

volume = "8",

pages = "93--97",

journal = "Drug Metabolism and Disposition",

issn = "0090-9556",

publisher = "American Society for Pharmacology and Experimental Therapeutics",

number = "2",

}

TY - JOUR

T1 - Glutathione-dependent dechlorination of chloramphenicol by cytosol of rat liver

AU - Martin, J. L.

AU - George, J. W.

AU - Pohl, L. R.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - The 100,000 g supernatant fraction (cytosol) of rat liver converts chloramphenicol (CAP, RNHCOCHCl2) into two products. Most of the enzyme activity is lost during dialysis of the enzyme preparation overnight, but is restored by addition of glutathione (GSH). Other thiols are not as effective as GSH in restoring the enzyme activity. The formation of the metabolites is not inhibited when incubations are performed under anaerobic conditions. The major metabolite was identified as CAP aldehyde (RNHCOCHO) whereas the minor metabolite was identified as an alkali-unstable derivative of CAP oxamic acid (RNHCOCOOH). Plausible pathways are discussed for the formation of these metabolites.

AB - The 100,000 g supernatant fraction (cytosol) of rat liver converts chloramphenicol (CAP, RNHCOCHCl2) into two products. Most of the enzyme activity is lost during dialysis of the enzyme preparation overnight, but is restored by addition of glutathione (GSH). Other thiols are not as effective as GSH in restoring the enzyme activity. The formation of the metabolites is not inhibited when incubations are performed under anaerobic conditions. The major metabolite was identified as CAP aldehyde (RNHCOCHO) whereas the minor metabolite was identified as an alkali-unstable derivative of CAP oxamic acid (RNHCOCOOH). Plausible pathways are discussed for the formation of these metabolites.

UR - http://www.scopus.com/inward/record.url?scp=0018894670&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018894670&partnerID=8YFLogxK

M3 - Article

C2 - 6103795

AN - SCOPUS:0018894670

SN - 0090-9556

VL - 8

SP - 93

EP - 97

JO - Drug Metabolism and Disposition

JF - Drug Metabolism and Disposition

IS - 2

ER -

Glutathione-dependent dechlorination of chloramphenicol by cytosol of rat liver

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this