Glutathione dependent control of protein disulfide-sulfhydryl content by subcellular fractions of hepatic tissue

John Tod Isaacs, Francis Binkley

Research output: Contribution to journalArticle

Abstract

The disulfide-sulfhydryl (SS/SH) ratios of subcellular fractions of rat hepatic tissues were found to vary diurnally with the ratio lowest in the early morning and highest in the early evening. These changes were found in the nuclear, microsomal and cytosol fractions. The primary reaction is the reversible formation of mixed disulfides of glutathione with proteins. This formation is controlled by the activity of thiol transferase and the level of oxidized glutathione (GSSG) as substrate. Several enzymes including mitochondrial and microsomal oxidases, glutathione reductase and peroxidase and glucose-6-phosphate dehydrogenase were found to control the levels of GSSG. An NADPH-dependent microsomal oxidase system, inhibited by GSSG, was found to produce activated oxygen which served as substrate for glutathione peroxidase. Evidence is presented for the concept that the formation of mixed disulfides of proteins with glutathione is a mechanism for maintenance of a disulfide-sulfhydryl ratio such that the integrity of particulate membranes is maintained during oxidative and reductive stresses on the hepatic cells.

Original languageEnglish (US)
Pages (from-to)192-204
Number of pages13
JournalBiochimica et Biophysica Acta - General Subjects
Volume497
Issue number1
DOIs
StatePublished - Mar 29 1977
Externally publishedYes

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Subcellular Fractions
Glutathione Disulfide
Disulfides
Glutathione
Tissue
Liver
Glutathione Peroxidase
Proteins
Oxidoreductases
Concept Formation
Glutathione Reductase
Glucosephosphate Dehydrogenase
Substrates
Transferases
NADP
Sulfhydryl Compounds
Cytosol
Rats
Hepatocytes
Oxidative Stress

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

Glutathione dependent control of protein disulfide-sulfhydryl content by subcellular fractions of hepatic tissue. / Isaacs, John Tod; Binkley, Francis.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 497, No. 1, 29.03.1977, p. 192-204.

Research output: Contribution to journalArticle

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