The plasma desorption mass spectra of large peptides, dissolved and electrosprayed in solutions containing glutathione, show increased molecular ion signal, reduction of base-line noise and peak widths, and an increase In multiply charged ions. The reduced, rather than the oxidized, form of glutathione is responsible for these effects. Some other chemically similar matrices show similar effects while others do not. Several roles for the matrix are suggested including previously reported effects on protein refolding and aggregation in solution, as well as possibilities for lowering the sample/substrate binding energy during desorption.
ASJC Scopus subject areas
- Analytical Chemistry