Glutamate transport in cultures from developing avian cerebellum: Presence of GLT-1 immunoreactivity in Purkinje neurons

Jennifer A. Meaney, Vladimir J. Balcar, Jeffrey D. Rothstein, Peter L. Jeffrey

Research output: Contribution to journalArticlepeer-review

Abstract

Immunocytochemical studies indicated that Purkinje cells cultured from chick embryonic cerebellum (embryonic day 8) strongly express a glutamate transporter EAAT2 cloned from human brain (GLT-1 in rat brain). At both 7 days and 14 days in culture, Purkinje neurons accumulated 1 μM [3H]L- glutamate via a potent 'high-affinity' transport system that could be inhibited by D- and L-threo-3-hydroxyaspartate (D- and L-t-3OHA) and by L- trans-pyrrolidine-2,4-dicarboxylate (L-t-PDC). The order of potency of the three inhibitors was L-t-PDC ~ L-t-3OHA > D-t-3OHA. Only the value of IC50 (concentration causing 50% inhibition) for D-t-3OHA significantly changed between 7 days (116 μ) and 14 days in culture (40 μM). All n(H) ~ 1, except in the case of the inhibition by D-t-3OHA at 14 days in culture (n(H) = 0.57), indicating the possible appearance of heterogeneity of the transport sites at later stages of culturing. Chronic inhibition of L-glutamate transport by L-t-PDC resulted in major changes in the morphology of Purkinje cells; particularly, the neurites almost completely regressed.

Original languageEnglish (US)
Pages (from-to)595-603
Number of pages9
JournalJournal of neuroscience research
Volume54
Issue number5
DOIs
StatePublished - Dec 1 1998

Keywords

  • Chick cerebellum
  • D- and L-threo-3-hydroxyaspartate
  • Excitatory amino acids
  • GLT-1
  • Growth of neurites
  • L- trans-pyrrolidine-2,4-dicarboxylate

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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