Glutamate receptor binding in juvenile and adult Rana pipiens CNS

Hollis T. Cline, J. W. McDonald, Martha Constantine‐Paton

Research output: Contribution to journalArticlepeer-review

Abstract

Autoradiographic methods were used to map NMDA and quisqualate‐sensitive glutamate binding sites in the brain of mature and juvenile Rana pipiens frogs. NMDA and quisqualate‐sensitive sites were consistently co‐localized in the CNS. The highest glutamate binding occurred in the telencephalon, hypothalamus, and cerebellum. Glutamate binding sites were also specifical neuropil of the optic tectum, consistent with glutamate being the retinal ganglion cell neurotransmitter. The distribution of glutamate binding sites in the brain of juvenile postmate morphic frogs was similar to that in adults. In general, Quis binding increased about twofold in adults compared to juveniles, whereas NMDA binding did not show a comparable developmental increase. To test whether glutamate binding sites are located on retinal axon terminals or on tectal cell dendrites in the optic tectum, juvenile postmetamorphic frogs were enucleated unilaterally, and receptor binding was performed following 1, 3, 7, and 14 days survival. The denervated tectal neuropil showed a delayed decrease in NMDA‐and quiequalate‐sensitive binding, consistent with the receptors being located on postsynaptic tectal cell dendrites. © 1994 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)488-502
Number of pages15
JournalJournal of Neurobiology
Volume25
Issue number5
DOIs
StatePublished - May 1994

Keywords

  • NMDA
  • Rana pipiens CNS
  • glutamate
  • receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

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