Glutamate receptor binding in juvenile and adult Rana pipiens CNS

H. T. Cline, J. W. McDonald, M. Constantine-Paton

Research output: Contribution to journalArticle

Abstract

Autoradiographic methods were used to map NMDA- and quisqualate-sensitive glutamate binding sites in the brain of mature and juvenile Rana pipiens frogs. NMDA- and quisqualate-sensitive sites were consistently co-localized in the CNS. The highest glutamate binding occurred in the telencephalon, hypothalamus, and cerebellum. Glutamate binding sites were also specifically localized in visual pathways, including the superficial neuropil of the optic tectum, consistent with glutamate being the retinal ganglion cell neurotransmitter. The distribution of glutamate binding sites in the brain of juvenile postmetamorphic frogs was similar to that in adults. In general, Quis binding increased about twofold in adults compared to juveniles, whereas NMDA binding did not show a comparable developmental increase. To test whether glutamate binding sites are located on retinal axon terminals or on tectal cell dendrites in the optic tectum, juvenile postmetamorphic frogs were enucleated unilaterally, and receptor binding was performed following 1, 3, 7, and 14 days survival. The denervated tectal neuropil showed a delayed decrease in NMDA- and quisqualate-sensitive binding, consistent with the receptors being located on postsynaptic tectal cell dendrites.

Original languageEnglish (US)
Pages (from-to)488-502
Number of pages15
JournalJournal of Neurobiology
Volume25
Issue number5
StatePublished - 1994
Externally publishedYes

Fingerprint

Rana pipiens
Glutamate Receptors
Glutamic Acid
N-Methylaspartate
Quisqualic Acid
Binding Sites
Anura
Neuropil
Superior Colliculi
Dendrites
Telencephalon
Visual Pathways
Retinal Ganglion Cells
Presynaptic Terminals
Brain
Cerebellum
Hypothalamus
Neurotransmitter Agents

Keywords

  • glutamate
  • NMDA
  • Rana pipiens CNS
  • receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cline, H. T., McDonald, J. W., & Constantine-Paton, M. (1994). Glutamate receptor binding in juvenile and adult Rana pipiens CNS. Journal of Neurobiology, 25(5), 488-502.

Glutamate receptor binding in juvenile and adult Rana pipiens CNS. / Cline, H. T.; McDonald, J. W.; Constantine-Paton, M.

In: Journal of Neurobiology, Vol. 25, No. 5, 1994, p. 488-502.

Research output: Contribution to journalArticle

Cline, HT, McDonald, JW & Constantine-Paton, M 1994, 'Glutamate receptor binding in juvenile and adult Rana pipiens CNS', Journal of Neurobiology, vol. 25, no. 5, pp. 488-502.
Cline HT, McDonald JW, Constantine-Paton M. Glutamate receptor binding in juvenile and adult Rana pipiens CNS. Journal of Neurobiology. 1994;25(5):488-502.
Cline, H. T. ; McDonald, J. W. ; Constantine-Paton, M. / Glutamate receptor binding in juvenile and adult Rana pipiens CNS. In: Journal of Neurobiology. 1994 ; Vol. 25, No. 5. pp. 488-502.
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