Glutamate mediates platelet activation through the AMPA receptor

Craig N. Morrell, Henry Sun, Masahiro Ikeda, Jean Claude Beique, Anne Marie Swaim, Emily Mason, Tanika V. Martin, Laura E. Thompson, Oguz Gozen, David Ampagoomian, Rolf Sprengel, Jeffrey Rothstein, Nauder Faraday, Richard Huganir, Charles J. Lowenstein

Research output: Contribution to journalArticle

Abstract

Glutamate is an excitatory neurotransmitter that binds to the kainate receptor, the N -methyl-D-aspartate (NMDA) receptor, and the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor (AMPAR). Each receptor was first characterized and cloned in the central nervous system (CNS). Glutamate is also present in the periphery, and glutamate receptors have been identified in nonneuronal tissues, including bone, heart, kidney, pancreas, and platelets. Platelets play a central role in normal thrombosis and hemostasis, as well as contributing greatly to diseases such as stroke and myocardial infarction. Despite the presence of glutamate in platelet granules, the role of glutamate during hemostasis is unknown. We now show that activated platelets release glutamate, that platelets express AMPAR subunits, and that glutamate increases agonist-induced platelet activation. Furthermore, we demonstrate that glutamate binding to the AMPAR increases intracellular sodium concentration and depolarizes platelets, which are important steps in platelet activation. In contrast, platelets treated with the AMPAR antagonist CNQX or platelets derived from GluR1 knockout mice are resistant to AMPA effects. Importantly, mice lacking GluR1 have a prolonged time to thrombosis in vivo. Our data identify glutamate as a regulator of platelet activation, and suggest that the AMPA receptor is a novel antithrombotic target. JEM

Original languageEnglish (US)
Pages (from-to)575-584
Number of pages10
JournalJournal of Experimental Medicine
Volume205
Issue number3
DOIs
StatePublished - Mar 17 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Morrell, C. N., Sun, H., Ikeda, M., Beique, J. C., Swaim, A. M., Mason, E., Martin, T. V., Thompson, L. E., Gozen, O., Ampagoomian, D., Sprengel, R., Rothstein, J., Faraday, N., Huganir, R., & Lowenstein, C. J. (2008). Glutamate mediates platelet activation through the AMPA receptor. Journal of Experimental Medicine, 205(3), 575-584. https://doi.org/10.1084/jem.20071474