Glucosephosphate isomerase distribution during oocyte maturation and early embryologic stages of the mouse

J. Gearhart, M. L. Oster-Granite

Research output: Contribution to journalArticle

Abstract

The enzyme glucosephosphate isomerase (GPI) is of interest because of its role in glycolysis and its use as a cell marker in studies of embryogenesis utilizing chimeras. The distribution of GPI was determined in histologic sections of ovarian follicles and the tissues of early embryonic stages of the mouse. Antibodies of GPI were combined in an immunocytochemical procedure utilizing peroxidase-antiperoxidase to localize GPI in tissue sections. During follicular maturation, GPI is first detected in oocytes of small follicles and then in the granulosa cells of slightly larger folicles. The relative concentrations of GPI remain high in the granulosa cells adjacent to the oocyte as the follicles mature. GPI in the granulosa cells may play a role in providing the growing oocyte with low molecular weight energy sources. Within the conceptus, differences in GPI concentrations among tissues become apparent during the formation of the egg cylinder. The highest concentrations of GPI are in the embryonic ectoderm, the amniotic folds, the extraembryonic ectoderm, and the ectoplacental cone. These are areas of intense mitotic activity. The energy required for cell divisions during embryogenesis is derived from glycolysis, thus the high concentrations of GPI in these tissues correlates well with the cellular function.

Original languageEnglish (US)
Pages (from-to)837-845
Number of pages9
JournalBiology of Reproduction
Volume23
Issue number4
StatePublished - 1980
Externally publishedYes

Fingerprint

Glucose-6-Phosphate Isomerase
Oocytes
Granulosa Cells
Ectoderm
Glycolysis
Embryonic Development
Ovarian Follicle
Cell Division
Peroxidase
Ovum
Molecular Weight

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Glucosephosphate isomerase distribution during oocyte maturation and early embryologic stages of the mouse. / Gearhart, J.; Oster-Granite, M. L.

In: Biology of Reproduction, Vol. 23, No. 4, 1980, p. 837-845.

Research output: Contribution to journalArticle

@article{09b59acd1ace402b9d1e6cb4de976949,
title = "Glucosephosphate isomerase distribution during oocyte maturation and early embryologic stages of the mouse",
abstract = "The enzyme glucosephosphate isomerase (GPI) is of interest because of its role in glycolysis and its use as a cell marker in studies of embryogenesis utilizing chimeras. The distribution of GPI was determined in histologic sections of ovarian follicles and the tissues of early embryonic stages of the mouse. Antibodies of GPI were combined in an immunocytochemical procedure utilizing peroxidase-antiperoxidase to localize GPI in tissue sections. During follicular maturation, GPI is first detected in oocytes of small follicles and then in the granulosa cells of slightly larger folicles. The relative concentrations of GPI remain high in the granulosa cells adjacent to the oocyte as the follicles mature. GPI in the granulosa cells may play a role in providing the growing oocyte with low molecular weight energy sources. Within the conceptus, differences in GPI concentrations among tissues become apparent during the formation of the egg cylinder. The highest concentrations of GPI are in the embryonic ectoderm, the amniotic folds, the extraembryonic ectoderm, and the ectoplacental cone. These are areas of intense mitotic activity. The energy required for cell divisions during embryogenesis is derived from glycolysis, thus the high concentrations of GPI in these tissues correlates well with the cellular function.",
author = "J. Gearhart and Oster-Granite, {M. L.}",
year = "1980",
language = "English (US)",
volume = "23",
pages = "837--845",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "4",

}

TY - JOUR

T1 - Glucosephosphate isomerase distribution during oocyte maturation and early embryologic stages of the mouse

AU - Gearhart, J.

AU - Oster-Granite, M. L.

PY - 1980

Y1 - 1980

N2 - The enzyme glucosephosphate isomerase (GPI) is of interest because of its role in glycolysis and its use as a cell marker in studies of embryogenesis utilizing chimeras. The distribution of GPI was determined in histologic sections of ovarian follicles and the tissues of early embryonic stages of the mouse. Antibodies of GPI were combined in an immunocytochemical procedure utilizing peroxidase-antiperoxidase to localize GPI in tissue sections. During follicular maturation, GPI is first detected in oocytes of small follicles and then in the granulosa cells of slightly larger folicles. The relative concentrations of GPI remain high in the granulosa cells adjacent to the oocyte as the follicles mature. GPI in the granulosa cells may play a role in providing the growing oocyte with low molecular weight energy sources. Within the conceptus, differences in GPI concentrations among tissues become apparent during the formation of the egg cylinder. The highest concentrations of GPI are in the embryonic ectoderm, the amniotic folds, the extraembryonic ectoderm, and the ectoplacental cone. These are areas of intense mitotic activity. The energy required for cell divisions during embryogenesis is derived from glycolysis, thus the high concentrations of GPI in these tissues correlates well with the cellular function.

AB - The enzyme glucosephosphate isomerase (GPI) is of interest because of its role in glycolysis and its use as a cell marker in studies of embryogenesis utilizing chimeras. The distribution of GPI was determined in histologic sections of ovarian follicles and the tissues of early embryonic stages of the mouse. Antibodies of GPI were combined in an immunocytochemical procedure utilizing peroxidase-antiperoxidase to localize GPI in tissue sections. During follicular maturation, GPI is first detected in oocytes of small follicles and then in the granulosa cells of slightly larger folicles. The relative concentrations of GPI remain high in the granulosa cells adjacent to the oocyte as the follicles mature. GPI in the granulosa cells may play a role in providing the growing oocyte with low molecular weight energy sources. Within the conceptus, differences in GPI concentrations among tissues become apparent during the formation of the egg cylinder. The highest concentrations of GPI are in the embryonic ectoderm, the amniotic folds, the extraembryonic ectoderm, and the ectoplacental cone. These are areas of intense mitotic activity. The energy required for cell divisions during embryogenesis is derived from glycolysis, thus the high concentrations of GPI in these tissues correlates well with the cellular function.

UR - http://www.scopus.com/inward/record.url?scp=0019278391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019278391&partnerID=8YFLogxK

M3 - Article

C2 - 7448284

AN - SCOPUS:0019278391

VL - 23

SP - 837

EP - 845

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 4

ER -