Glucosephosphate isomerase distribution during oocyte maturation and early embryologic stages of the mouse

J. Gearhart, M. L. Oster-Granite

Research output: Contribution to journalArticle

Abstract

The enzyme glucosephosphate isomerase (GPI) is of interest because of its role in glycolysis and its use as a cell marker in studies of embryogenesis utilizing chimeras. The distribution of GPI was determined in histologic sections of ovarian follicles and the tissues of early embryonic stages of the mouse. Antibodies of GPI were combined in an immunocytochemical procedure utilizing peroxidase-antiperoxidase to localize GPI in tissue sections. During follicular maturation, GPI is first detected in oocytes of small follicles and then in the granulosa cells of slightly larger folicles. The relative concentrations of GPI remain high in the granulosa cells adjacent to the oocyte as the follicles mature. GPI in the granulosa cells may play a role in providing the growing oocyte with low molecular weight energy sources. Within the conceptus, differences in GPI concentrations among tissues become apparent during the formation of the egg cylinder. The highest concentrations of GPI are in the embryonic ectoderm, the amniotic folds, the extraembryonic ectoderm, and the ectoplacental cone. These are areas of intense mitotic activity. The energy required for cell divisions during embryogenesis is derived from glycolysis, thus the high concentrations of GPI in these tissues correlates well with the cellular function.

Original languageEnglish (US)
Pages (from-to)837-845
Number of pages9
JournalBiology of Reproduction
Volume23
Issue number4
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

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