Glucocorticoid hormones downregulate histidine decarboxylase mRNA and enzyme activity in rat lung

Cynthia Zahnow, Pertti Panula, Atsushi Yamatodani, David E. Millhorn

Research output: Contribution to journalArticle

Abstract

Histidine decarboxylase (HDC) is the primary enzyme regulating histamine biosynthesis. Histamine contributes to the pathogenesis of chronic inflammatory disorders such as asthma. Because glucocorticoids are effective in the treatment of asthma, we examined the effects of 6 h of exogenously administered dexamethasone (0.5-3,000 μg/kg ip), corticosterone (0.2-200 mg/kg ip), or endogenously elevated corticosterone (via exposure of rats to 10% oxygen) on HDC expression in the rat lung. HDC transcripts were decreased ~73% with dexamethasone treatment, 57% with corticosterone treatment, and 50% with exposure to 10% oxygen. Likewise, HDC enzyme activity was decreased 80% by treatment with dexamethasone and corticosterone and 60% by exposure to 10% oxygen. Adrenalectomy prevented the decreases in HDC mRNA and enzyme activity observed in rats exposed to 10% oxygen, suggesting that the adrenal gland is necessary for the mediation of hypoxic effects on HDC gene expression. These results demonstrate that corticosteroids initiate a process that leads to the decrease of HDC mRNA levels and enzyme activity in rat lung.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume275
Issue number2 19-2
StatePublished - Aug 1998
Externally publishedYes

Fingerprint

Histidine Decarboxylase
Glucocorticoids
Down-Regulation
Hormones
Lung
Messenger RNA
Corticosterone
Enzymes
Dexamethasone
Oxygen
Histamine
Asthma
Adrenalectomy
Adrenal Glands
Adrenal Cortex Hormones
Gene Expression

Keywords

  • Asthma
  • Corticosterone
  • Histamine
  • Histidine decarboxylase gene
  • Hypoxia

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Glucocorticoid hormones downregulate histidine decarboxylase mRNA and enzyme activity in rat lung. / Zahnow, Cynthia; Panula, Pertti; Yamatodani, Atsushi; Millhorn, David E.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 275, No. 2 19-2, 08.1998.

Research output: Contribution to journalArticle

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