GlucoCEST magnetic resonance imaging in vivo may be diagnostic of acute renal allograft rejection

Dominik Kentrup, Philipp Bovenkamp, Annika Busch, Katharina Schuette-Nuetgen, Helga Pawelski, Hermann Pavenstädt, Eberhard Schlatter, Karl Heinz Herrmann, Jürgen R. Reichenbach, Bettina Löffler, Barbara Heitplatz, Veerle Van Marck, Nirbhay N. Yadav, Guanshu Liu, Peter C.M. van Zijl, Stefan Reuter, Verena Hoerr

Research output: Contribution to journalArticle

Abstract

Acute cellular renal allograft rejection (AR) frequently occurs after kidney transplantations. It is a sterile T-cell mediated inflammation leading to increased local glucose metabolism. Here we demonstrate in an allogeneic model of Brown Norway rat kidneys transplanted into uninephrectomized Lewis rats the successful implementation of the recently developed glucose chemical exchange saturation transfer (glucoCEST) magnetic resonance imaging. This technique is a novel method to assess and differentiate AR. Renal allografts undergoing AR showed significantly increased glucoCEST contrast ratios of cortex to medulla of 1.61 compared to healthy controls (1.02), syngeneic Lewis kidney to Lewis rat transplants without rejection (0.92), kidneys with ischemia reperfusion injury (0.99) and kidneys affected by cyclosporine A toxicity (1.10). Receiver operating characteristic curve analysis showed an area under the curve value of 0.92, and the glucoCEST contrast ratio predicted AR with a sensitivity of 100% and a specificity of 69% at a threshold level over 1.08. In defined animal models of kidney injuries, the glucoCEST contrast ratios of cortex to medulla correlated positively with mRNA expression levels of T-cell markers (CD3, CD4, CD8a/b), but did not correlate to impaired renal perfusion. Thus, the glucoCEST parameter may be valuable for the assessment and follow up treatment of AR.

Original languageEnglish (US)
Pages (from-to)757-764
Number of pages8
JournalKidney International
Volume92
Issue number3
DOIs
StatePublished - Sep 1 2017

Fingerprint

Allografts
Magnetic Resonance Imaging
Kidney
Glucose
T-Lymphocytes
Graft Rejection
Reperfusion Injury
ROC Curve
Kidney Transplantation
Cyclosporine
Area Under Curve
Inflammation
Messenger RNA
Wounds and Injuries

Keywords

  • acute rejection
  • glucoCEST MRI
  • inflammation
  • kidney transplantation

ASJC Scopus subject areas

  • Medicine(all)
  • Nephrology

Cite this

Kentrup, D., Bovenkamp, P., Busch, A., Schuette-Nuetgen, K., Pawelski, H., Pavenstädt, H., ... Hoerr, V. (2017). GlucoCEST magnetic resonance imaging in vivo may be diagnostic of acute renal allograft rejection. Kidney International, 92(3), 757-764. DOI: 10.1016/j.kint.2017.04.015

GlucoCEST magnetic resonance imaging in vivo may be diagnostic of acute renal allograft rejection. / Kentrup, Dominik; Bovenkamp, Philipp; Busch, Annika; Schuette-Nuetgen, Katharina; Pawelski, Helga; Pavenstädt, Hermann; Schlatter, Eberhard; Herrmann, Karl Heinz; Reichenbach, Jürgen R.; Löffler, Bettina; Heitplatz, Barbara; Van Marck, Veerle; Yadav, Nirbhay N.; Liu, Guanshu; van Zijl, Peter C.M.; Reuter, Stefan; Hoerr, Verena.

In: Kidney International, Vol. 92, No. 3, 01.09.2017, p. 757-764.

Research output: Contribution to journalArticle

Kentrup, D, Bovenkamp, P, Busch, A, Schuette-Nuetgen, K, Pawelski, H, Pavenstädt, H, Schlatter, E, Herrmann, KH, Reichenbach, JR, Löffler, B, Heitplatz, B, Van Marck, V, Yadav, NN, Liu, G, van Zijl, PCM, Reuter, S & Hoerr, V 2017, 'GlucoCEST magnetic resonance imaging in vivo may be diagnostic of acute renal allograft rejection' Kidney International, vol 92, no. 3, pp. 757-764. DOI: 10.1016/j.kint.2017.04.015
Kentrup D, Bovenkamp P, Busch A, Schuette-Nuetgen K, Pawelski H, Pavenstädt H et al. GlucoCEST magnetic resonance imaging in vivo may be diagnostic of acute renal allograft rejection. Kidney International. 2017 Sep 1;92(3):757-764. Available from, DOI: 10.1016/j.kint.2017.04.015

Kentrup, Dominik; Bovenkamp, Philipp; Busch, Annika; Schuette-Nuetgen, Katharina; Pawelski, Helga; Pavenstädt, Hermann; Schlatter, Eberhard; Herrmann, Karl Heinz; Reichenbach, Jürgen R.; Löffler, Bettina; Heitplatz, Barbara; Van Marck, Veerle; Yadav, Nirbhay N.; Liu, Guanshu; van Zijl, Peter C.M.; Reuter, Stefan; Hoerr, Verena / GlucoCEST magnetic resonance imaging in vivo may be diagnostic of acute renal allograft rejection.

In: Kidney International, Vol. 92, No. 3, 01.09.2017, p. 757-764.

Research output: Contribution to journalArticle

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