Glucagon-like peptide 2 stimulates intestinal epithelial proliferation in vitro

Jasleen Jasleen, Stanley W. Ashley, Naoshi Shimoda, Michael J. Zinner, Edward E. Whang

Research output: Contribution to journalArticlepeer-review

Abstract

Glucagon-like peptide 2 (GLP-2) is a potent intestine-specific growth factor. To determine whether GLP-2 has a direct effect on cellular proliferation, we examined the effects of GLP-2 on three different intestinal epithelial cell lines: Caco-2, T84, and IEC-6. Proliferation was assessed by measuring [3H]thymidine incorporation into cellular DNA. The expression of the cell cycle regulators cyclins D1, E, and A was determined by western blotting. GLP-2 stimulated [3H]thymidine incorporation in Caco-2 and T84 cells but not in IEC-6 cells. In Caco-2 cells, increased [3H]thymidine incorporation was detectable by day 1 and was maximal at day 5 following initiation of daily GLP-2 administration. Increased [3H]thymidine incorporation persisted for three days following treatment with a single dose of GLP-2. GLP-2 administration resulted in the induction of cyclins D1, E, and A in Caco-2 cells. These results suggest that GLP-2 acts directly on human intestinal epithelial cells to stimulate their proliferation.

Original languageEnglish (US)
Pages (from-to)1135-1140
Number of pages6
JournalDigestive diseases and sciences
Volume47
Issue number5
DOIs
StatePublished - 2002

Keywords

  • Cell cycle
  • Cyclins
  • Enteroglucagon
  • Glucagon-like peptide 2
  • Intestinal adaptation
  • Intestinal regeneration

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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