Glucagon-like peptide-1 does not mediate amylase release from AR42J cells

J. Zhou, C. Montrose-Rafizadeh, A. M. Janczewski, M. A. Pineyro, S. J. Sollott, Y. Wang, J. M. Egan

Research output: Contribution to journalArticle

Abstract

In this study, AR42J pancreatic acinar cells were used to investigate if glucagon-like peptide-1 (GLP-1) or glucagon might influence amylase release and acinar cell function. We first confirmed the presence of GLP-1 receptors on AR42J cells by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting, and partial sequencing analysis. While cholecystokinin (CCK) increased amylase release from AR42J cells, GLP-1, alone or in the presence of CCK, had no effect on amylase release but both CCK and GLP-1 increased intracellular calcium. Similar to GLP-1, glucagon increased both cyclic adenosine monophosphate (cAMP) and intracellular calcium in AR42J cells but it actually decreased CCK-mediated amylase release (n = 20, P <0.01). CCK stimulation resulted in an increase in tyrosine phosphorylation of several cellular proteins, unlike GLP-1 treatment, where no such increased phosphorylation was seen. Instead, GLP-1 decreased such protein phosphorylations. Genestein blocked CCK-induced phosphorylation events and amylase secretion while vanadate increased amylase secretion. These results provide evidence that tyrosine phosphorylation is necessary for amylase release and that signaling through GLP-1 receptors does not mediate amylase release in AR42J cells.

Original languageEnglish (US)
Pages (from-to)470-478
Number of pages9
JournalJournal of Cellular Physiology
Volume181
Issue number3
DOIs
StatePublished - 1999
Externally publishedYes

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Glucagon-Like Peptide 1
Amylases
Cholecystokinin
Phosphorylation
Acinar Cells
Glucagon
Tyrosine
Calcium
Vanadates
Genistein
Polymerase chain reaction
RNA-Directed DNA Polymerase
Reverse Transcriptase Polymerase Chain Reaction
Cyclic AMP
Proteins
Western Blotting

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Zhou, J., Montrose-Rafizadeh, C., Janczewski, A. M., Pineyro, M. A., Sollott, S. J., Wang, Y., & Egan, J. M. (1999). Glucagon-like peptide-1 does not mediate amylase release from AR42J cells. Journal of Cellular Physiology, 181(3), 470-478. https://doi.org/10.1002/(SICI)1097-4652(199912)181:3<470::AID-JCP11>3.0.CO;2-P

Glucagon-like peptide-1 does not mediate amylase release from AR42J cells. / Zhou, J.; Montrose-Rafizadeh, C.; Janczewski, A. M.; Pineyro, M. A.; Sollott, S. J.; Wang, Y.; Egan, J. M.

In: Journal of Cellular Physiology, Vol. 181, No. 3, 1999, p. 470-478.

Research output: Contribution to journalArticle

Zhou, J, Montrose-Rafizadeh, C, Janczewski, AM, Pineyro, MA, Sollott, SJ, Wang, Y & Egan, JM 1999, 'Glucagon-like peptide-1 does not mediate amylase release from AR42J cells', Journal of Cellular Physiology, vol. 181, no. 3, pp. 470-478. https://doi.org/10.1002/(SICI)1097-4652(199912)181:3<470::AID-JCP11>3.0.CO;2-P
Zhou, J. ; Montrose-Rafizadeh, C. ; Janczewski, A. M. ; Pineyro, M. A. ; Sollott, S. J. ; Wang, Y. ; Egan, J. M. / Glucagon-like peptide-1 does not mediate amylase release from AR42J cells. In: Journal of Cellular Physiology. 1999 ; Vol. 181, No. 3. pp. 470-478.
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