GluA2-containing AMPA receptors distinguish ribbon-associated from ribbonless afferent contacts on rat cochlear hair cells

Rodrigo Martinez-Monedero, Chang Liu, Catherine Weisz, Pankhuri Vyas, Paul Albert Fuchs, Elisabeth B Glowatzki

Research output: Contribution to journalArticle

Abstract

Mechanosensory hair cells release glutamate at ribbon synapses to excite postsynaptic afferent neurons, via AMPA-type ionotropic glutamate receptors (AMPARs). However, type II afferent neurons contacting outer hair cells in the mammalian cochlea were thought to differ in this respect, failing to show GluA immunolabeling and with many "ribbonless" afferent contacts. Here it is shown that antibodies to the AMPAR subunit GluA2 labeled afferent contacts below inner and outer hair cells in the rat cochlea, and that synaptic currents in type II afferents had AMPAR-specific pharmacology. Only half the postsynaptic densities of type II afferents that labeled for PSD-95, Shank, or Homer were associated with GluA2 immunopuncta or presynaptic ribbons, the "empty slots" corresponding to ribbonless contacts described previously. These results extend the universality of AMPAergic transmission by hair cells, and support the existence of silent afferent contacts.

Original languageEnglish (US)
Pages (from-to)11080-11085
Number of pages6
JournaleNeuro
Volume3
Issue number2
DOIs
StatePublished - 2016

    Fingerprint

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this