GLT-1 promoter activity in astrocytes and neurons of mouse hippocampus and somatic sensory cortex

Luisa de Vivo, Marcello Melone, Jeffrey D. Rothstein, Fiorenzo Conti

Research output: Contribution to journalArticle

Abstract

GLT-1 eGFP BAC reporter transgenic adult mice were used to detect GLT-1 gene expression in individual cells of CA1, CA3 and SI, and eGFP fl uorescence was measured to analyze quantitatively GLT-1 promoter activity in different cells of neocortex and hippocampus. Virtually all GFAP+ astrocytes were eGFP+; we also found that about 80% of neurons in CA3 pyramidal layer, 10-70% of neurons in I-VI layers of SI and rare neurons in all strata of CA1 and in strata oriens and radiatum of CA3 were eGFP+. Analysis of eGFP intensity showed that astrocytes had a higher GLT-1 promoter activity in SI than in CA1 and CA3, and that neurons had the highest levels of GLT-1 promoter activity in CA3 stratum pyramidale and in layer VI of SI. Finally, we observed that the intensity of GLT-1 promoter activity in neurons is 1-20% of that measured in astrocytes. These results showed that in the hippocampus and neocortex GLT-1 promoter activity is observed in astrocytes and neurons, detailed the distribution of GLT-1 expressing neurons, and indicated that GLT-1 promoter activity in both astrocytes and neurons varies in different brain regions.

Original languageEnglish (US)
Article number31
JournalFrontiers in Neuroanatomy
Volume3
Issue numberJAN
DOIs
StatePublished - Jan 11 2010

Keywords

  • Astrocytes
  • GLT-1/EAAT2
  • Glutamate transporters
  • Hippocampus
  • Neocortex
  • Neurons

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience (miscellaneous)
  • Cellular and Molecular Neuroscience

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