Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions

Narie Sasaki, Haruko Kuroiwa, Chikako Nishitani, Hiroyoshi Takano, Tetsuya Higashiyama, Tamaki Kobayashi, Yuki Shirai, Atsushi Sakai, Shigeyuki Kawano, Kimiko Murakami-Murofushi, Tsuneyoshi Kuroiwa

Research output: Contribution to journalArticle

Abstract

Mitochondrial DNA (mtDNA) is packed into highly organized structures called mitochondrial nucleoids (mt-nucleoids). To understand the organization of mtDNA and the overall regulation of its genetic activity within the mt-nucleoids, we identified and characterized a novel mtDNA packaging protein, termed Glom (a protein inducing agglomeration of mitochondrial chromosome), from highly condensed mt-nucleoids of the true slime mold, Physarum polycephalum. This protein could bind to the entire mtDNA and package mtDNA into a highly condensed state in vitro. Immunostaining analysis showed that Glom specifically localized throughout the mt-nucleoid. Deduced amino acid sequence revealed that Glom has a lysine-rich region with proline-rich domain in the N-terminal half and two HMG boxes in C-terminal half. Deletion analysis of Glom revealed that the lysine-rich region was sufficient for the intense mtDNA condensation in vitro. When the recombinant Glom proteins containing the lysine-rich region were expressed in Escherichia coli, the condensed nucleoid structures were observed in E. coli. Such in vivo condensation did not interfere with transcription or replication of E. coli chromosome and the proline-rich domain was essential to keep those genetic activities. The expression of Glom also complemented the E. coli mutant lacking the bacterial histone-like protein HU and the HMG-boxes region of Glom was important for the complementation. Our results suggest that Glom is a new mitochondrial histone-like protein having a property to cause intense DNA condensation without suppressing DNA functions.

Original languageEnglish (US)
Pages (from-to)4758-4769
Number of pages12
JournalMolecular Biology of the Cell
Volume14
Issue number12
DOIs
StatePublished - Dec 2003
Externally publishedYes

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DNA Packaging
Physarum polycephalum
Mitochondrial DNA
Chromatin
DNA
Escherichia coli
Lysine
Proteins
Proline
Histones
HMGB Proteins
Chromosomes
Myxomycetes
Recombinant Proteins
Amino Acid Sequence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions. / Sasaki, Narie; Kuroiwa, Haruko; Nishitani, Chikako; Takano, Hiroyoshi; Higashiyama, Tetsuya; Kobayashi, Tamaki; Shirai, Yuki; Sakai, Atsushi; Kawano, Shigeyuki; Murakami-Murofushi, Kimiko; Kuroiwa, Tsuneyoshi.

In: Molecular Biology of the Cell, Vol. 14, No. 12, 12.2003, p. 4758-4769.

Research output: Contribution to journalArticle

Sasaki, N, Kuroiwa, H, Nishitani, C, Takano, H, Higashiyama, T, Kobayashi, T, Shirai, Y, Sakai, A, Kawano, S, Murakami-Murofushi, K & Kuroiwa, T 2003, 'Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions', Molecular Biology of the Cell, vol. 14, no. 12, pp. 4758-4769. https://doi.org/10.1091/mbc.E03-02-0099
Sasaki, Narie ; Kuroiwa, Haruko ; Nishitani, Chikako ; Takano, Hiroyoshi ; Higashiyama, Tetsuya ; Kobayashi, Tamaki ; Shirai, Yuki ; Sakai, Atsushi ; Kawano, Shigeyuki ; Murakami-Murofushi, Kimiko ; Kuroiwa, Tsuneyoshi. / Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions. In: Molecular Biology of the Cell. 2003 ; Vol. 14, No. 12. pp. 4758-4769.
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