Abstract
Friend erythroleukemia cells, originally derived from DBA/2 mice, differentiate when cultured with inducing agents. Studies of the different effects of inducing agents on clone 745 have revealed that both dimethyl sulfoxide (DMSO) and hemin produce benzidine-positive cells. Butyric acid produced mature but benzidine-negative cells in this clone. All agents induced globin synthesis above the 0.1% of protein synthesis found in uninduced cells. DMSO induction stimulated globin synthesis 9%, hemin 2%, and butyric acid 3%. Total β/α ratios were approximately unity with all agents. Although the inducing agents all stimulated total globin synthesis in Friend cells, the relative rates of synthesis of the two mouse β chains were affected differently by the various agents. Hemin markedly increased the proportion of β minor. For example, DBA/2 mouse reticulocytes synthesized 20% β minor and 80% β major. DMSO induction of clone 745 caused 20%-33% synthesis of β minor, and butyric acid 30%-37% β minor. In contrast, hemin increased the proportion of β minor to 64%-69%. Thus the Friend erythroleukemia cell system provides an in vitro approach to the study of the regulation of globin-chain switching.
Original language | English (US) |
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Pages (from-to) | 867-876 |
Number of pages | 10 |
Journal | Blood |
Volume | 50 |
Issue number | 5 |
State | Published - 1977 |
Externally published | Yes |
ASJC Scopus subject areas
- Hematology