Global survey of diversity among environmental saltwater Bacteriovoracaceae

Silvia A. Pineiro, O. Colin Stine, Ashvini Chauhan, Susan R. Steyert, Richard Smith, Henry N. Williams

Research output: Contribution to journalArticle

Abstract

Halophilic Bacteriovorax (Bx), formerly known as the marine Bdellovibrio, are Gram-negative, predatory bacteria found in saltwater systems. To assess their genetic diversity and geographical occurrence, the small subunit rRNA (ssu-rRNA) gene sequences were analysed from 111 marine, salt lake and estuarine isolates recovered from 27 locations around the world. Phylogenetic analysis of these isolates using Geobacter as the outgroup revealed eight distinct ribotype clusters each with at least two isolates. Each cluster was composed of isolates with ≥ 96.5% similarity in ssu-rRNA sequences. Three single isolate outliers were observed. Many of the Bx ribotypes were widely dispersed among different types of ecosystems (e.g. cluster III was recovered from the Great Salt Lake, the Atlantic Ocean, Pacific Ocean, Chesapeake Bay and gills of aquarium fish). However, cluster V was only recovered from a single ecosystem, estuaries. Cluster V was originally detected in the Chesapeake Bay and subsequently in the Pamlico Sound/Neuse River system. Principal coordinate analysis revealed that the sequences of the isolates from different environments were distinct from each other. The results of this study reveal the saltwater Bx to be phylogenetically and environmentally more diverse than was previously known.

Original languageEnglish (US)
Pages (from-to)2441-2450
Number of pages10
JournalEnvironmental Microbiology
Volume9
Issue number10
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

Bacteriovorax
Ribotyping
saline lake
Lakes
saline water
Ecosystem
ribotypes
Bdellovibrio
Geobacter
Salts
Chesapeake Bay
Atlantic Ocean
Pacific Ocean
Estuaries
ecosystem
ocean
aquarium
outlier
Gram-Negative Bacteria
rRNA Genes

ASJC Scopus subject areas

  • Environmental Science(all)
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Pineiro, S. A., Stine, O. C., Chauhan, A., Steyert, S. R., Smith, R., & Williams, H. N. (2007). Global survey of diversity among environmental saltwater Bacteriovoracaceae. Environmental Microbiology, 9(10), 2441-2450. https://doi.org/10.1111/j.1462-2920.2007.01362.x

Global survey of diversity among environmental saltwater Bacteriovoracaceae. / Pineiro, Silvia A.; Stine, O. Colin; Chauhan, Ashvini; Steyert, Susan R.; Smith, Richard; Williams, Henry N.

In: Environmental Microbiology, Vol. 9, No. 10, 10.2007, p. 2441-2450.

Research output: Contribution to journalArticle

Pineiro, SA, Stine, OC, Chauhan, A, Steyert, SR, Smith, R & Williams, HN 2007, 'Global survey of diversity among environmental saltwater Bacteriovoracaceae', Environmental Microbiology, vol. 9, no. 10, pp. 2441-2450. https://doi.org/10.1111/j.1462-2920.2007.01362.x
Pineiro, Silvia A. ; Stine, O. Colin ; Chauhan, Ashvini ; Steyert, Susan R. ; Smith, Richard ; Williams, Henry N. / Global survey of diversity among environmental saltwater Bacteriovoracaceae. In: Environmental Microbiology. 2007 ; Vol. 9, No. 10. pp. 2441-2450.
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