Global gene expression analysis reveals specific and redundant roles for H1 variants, H1c and H10, in gene expression regulation

Sheetal Bhan, Warren May, Stephanie L. Warren, Donald B. Sittman

Research output: Contribution to journalArticlepeer-review

Abstract

In mammals, the functional significance of the presence of evolutionarily conserved, multiple non-allelic H1 variants remains unclear. We used a unique overproduction approach coupled with cell cycle synchronization and early time point assays to assess differential effects of H1 variants, H1c and H10, on global gene expression in the absence of compensatory events that may mask variant-specific effects. We found that H1c and H10 act primarily as specific rather than global regulators of gene expression. Many of the genes affected were uniquely targeted by either H1c or H10, affirming that H1 variants have some unique roles. We also identified genes that were affected by both variants, in which cases the expression of these genes was, for the most part, affected similarly by both the variants. This observation suggests that as well as having specific functions, the H1 variants share common roles in the organization of chromatin. We further noted that H10 repressed more genes than did H1c, which may underlie the prevailing notion that H10 is a stronger repressor of transcription.

Original languageEnglish (US)
Pages (from-to)10-18
Number of pages9
JournalGene
Volume414
Issue number1-2
DOIs
StatePublished - May 15 2008

Keywords

  • Chromatin
  • Linker histone
  • Overexpression
  • Variants

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'Global gene expression analysis reveals specific and redundant roles for H1 variants, H1c and H1<sup>0</sup>, in gene expression regulation'. Together they form a unique fingerprint.

Cite this