Global diversity within and between human herpesvirus 1 and 2 glycoproteins

Susanna L. Lamers, Ruchi M. Newman, Oliver B. Laeyendecker, Aaron A Tobian, Robert C. Colgrove, Stuart Campbell Ray, David M. Koelle, Jeffrey Cohen, David M. Knipe, Thomas C Quinn

Research output: Contribution to journalArticle

Abstract

Human herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) are large-genome DNA viruses that establish a persistent infection in sensory neurons and commonly manifest with recurring oral or genital erosions that transmit virus. HSV encodes 12 predicted glycoproteins that serve various functions, including cellular attachment, entry, and egress. Glycoprotein G is currently the target of an antibody test to differentiate HSV-1 from HSV-2; however, this test has shown reduced capacity to differentiate HSV strains in East Africa. Until the recent availability of 26 full-length HSV-1 and 36 full-length HSV-2 sequences, minimal comparative information was available for these viruses. In this study, we use a variety of sequence analysis methods to compare all available sequence data for HSV-1 and HSV-2 glycoproteins, using viruses isolated in Europe, Asia, North America, the Republic of South Africa, and East Africa. We found numerous differences in diversity, nonsynonymous/synonymous substitution rates, and recombination rates between HSV-1 glycoproteins and their HSV-2 counterparts. Phylogenetic analysis revealed that while most global HSV-2 glycoprotein G sequences did not form clusters within or between continents, one clade (supported at 60.5%) contained 37% of the African sequences analyzed. Accordingly, sequences from this African subset contained unique amino acid signatures, not only in glycoprotein G, but also in glycoproteins I and E, which may account for the failure of sensitive antibody tests to distinguish HSV-1 from HSV-2 in some African individuals. Consensus sequences generated in the study can be used to improve diagnostic assays that differentiate HSV-1 from HSV-2 in global populations.

Original languageEnglish (US)
Pages (from-to)8206-8218
Number of pages13
JournalJournal of Virology
Volume89
Issue number16
DOIs
StatePublished - 2015

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Human herpesvirus 2
Human herpesvirus 1
Human Herpesvirus 2
Human Herpesvirus 1
glycoproteins
Glycoproteins
Eastern Africa
viruses
Viruses
DNA viruses
antibodies
sensory neurons
consensus sequence
testing
DNA Viruses
Antibodies
Consensus Sequence
Sensory Receptor Cells
genitalia
North America

ASJC Scopus subject areas

  • Immunology
  • Virology

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Global diversity within and between human herpesvirus 1 and 2 glycoproteins. / Lamers, Susanna L.; Newman, Ruchi M.; Laeyendecker, Oliver B.; Tobian, Aaron A; Colgrove, Robert C.; Ray, Stuart Campbell; Koelle, David M.; Cohen, Jeffrey; Knipe, David M.; Quinn, Thomas C.

In: Journal of Virology, Vol. 89, No. 16, 2015, p. 8206-8218.

Research output: Contribution to journalArticle

Lamers, Susanna L. ; Newman, Ruchi M. ; Laeyendecker, Oliver B. ; Tobian, Aaron A ; Colgrove, Robert C. ; Ray, Stuart Campbell ; Koelle, David M. ; Cohen, Jeffrey ; Knipe, David M. ; Quinn, Thomas C. / Global diversity within and between human herpesvirus 1 and 2 glycoproteins. In: Journal of Virology. 2015 ; Vol. 89, No. 16. pp. 8206-8218.
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