Global characterization and genomic stability of human multistem, a multipotent adult progenitor cell

Sherry Boozer, Nicholas Lehman, Uma Lakshmipathy, Brad Love, Amy Raber, Anirban Maitra, Robert Deans, Mahendra S. Rao, Anthony E. Ting

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The therapeutic benefits of adult adherent stem cells are currently being investigated in clinical trials for a variety of diseases. Data from initial clinical studies are promising and as a consequence of moving to later stage clinical studies, have resulted in larger scale clinical-grade cell production strategies. Therefore it becomes imperative to examine the epigenetic flux and genomic stability of stem cells in long-term culture to determine that minimal risk is associated with these therapies. Multipotent adult progenitor cells (MAPC) are an adherent adult stem cell population that can be derived from bone marrow and was the first of a class of adult stem cells that have broad developmental potential both in vitro and in vivo. Here, we report a panel of tests to characterize MultiStem, a multipotent adult stem cell type based on MAPC, and establish its genomic stability during culture expansion. A variety of techniques were employed that consisted of miRNA expression to characterize and define the cell population; chromosomal SNP analysis and G-banding to determine karyotypic stability; and methylation pattern and telomerase expression to examine potential changes in epigenetic and chromosomal stability with prolonged in vitro culture of cells. This panel of test was applied to cultures at early isolation stages and compared to cultures harvested at population doublings greater than those reached in current MultiStem clinical trials. These tests also provide a baseline for quality control of cells prepared from various biological donor sources for subsequent large scale propagation and preparation of cell banks for downstream applications.

Original languageEnglish (US)
Title of host publicationStem Cell Research Advancements
PublisherNova Science Publishers, Inc.
Pages119-134
Number of pages16
ISBN (Print)9781613240076
StatePublished - Mar 2011

Fingerprint

Adult Stem Cells
Genomic Instability
Stem Cells
Epigenomics
Clinical Trials
Multipotent Stem Cells
Population
Chromosomal Instability
Telomerase
MicroRNAs
Quality Control
Methylation
Single Nucleotide Polymorphism
Cell Culture Techniques
Bone Marrow
Therapeutics
Clinical Studies
In Vitro Techniques

Keywords

  • Adult stem cells
  • Gene methylation.
  • Genomic stability
  • MiRNA
  • Multipotent adult progenitor cells
  • MultiStem
  • SNP

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Boozer, S., Lehman, N., Lakshmipathy, U., Love, B., Raber, A., Maitra, A., ... Ting, A. E. (2011). Global characterization and genomic stability of human multistem, a multipotent adult progenitor cell. In Stem Cell Research Advancements (pp. 119-134). Nova Science Publishers, Inc..

Global characterization and genomic stability of human multistem, a multipotent adult progenitor cell. / Boozer, Sherry; Lehman, Nicholas; Lakshmipathy, Uma; Love, Brad; Raber, Amy; Maitra, Anirban; Deans, Robert; Rao, Mahendra S.; Ting, Anthony E.

Stem Cell Research Advancements. Nova Science Publishers, Inc., 2011. p. 119-134.

Research output: Chapter in Book/Report/Conference proceedingChapter

Boozer, S, Lehman, N, Lakshmipathy, U, Love, B, Raber, A, Maitra, A, Deans, R, Rao, MS & Ting, AE 2011, Global characterization and genomic stability of human multistem, a multipotent adult progenitor cell. in Stem Cell Research Advancements. Nova Science Publishers, Inc., pp. 119-134.
Boozer S, Lehman N, Lakshmipathy U, Love B, Raber A, Maitra A et al. Global characterization and genomic stability of human multistem, a multipotent adult progenitor cell. In Stem Cell Research Advancements. Nova Science Publishers, Inc. 2011. p. 119-134
Boozer, Sherry ; Lehman, Nicholas ; Lakshmipathy, Uma ; Love, Brad ; Raber, Amy ; Maitra, Anirban ; Deans, Robert ; Rao, Mahendra S. ; Ting, Anthony E. / Global characterization and genomic stability of human multistem, a multipotent adult progenitor cell. Stem Cell Research Advancements. Nova Science Publishers, Inc., 2011. pp. 119-134
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