Global analysis of stress-regulated mRNA turnover by using cDNA arrays

Jinshui Fan, Xiaoling Yang, Wengong Wang, William H. Wood, Kevin G. Becker, Myriam Gorospe

Research output: Contribution to journalArticlepeer-review

Abstract

cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the evaluation of the relative roles of transcription and mRNA turnover in governing gene expression on a global basis, compared with current individual gene-by-gene analyses. This method, which involves comparison of large-scale hybridization patterns generated with steady-state mRNA versus newly transcribed (nuclear run-on) RNA, was used to demonstrate the importance of mRNA turnover in regulating gene expression following several conditions of stress.

Original languageEnglish (US)
Pages (from-to)10611-10616
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number16
DOIs
StatePublished - Aug 2002
Externally publishedYes

Keywords

  • Gene expression
  • Stress response
  • Transcription

ASJC Scopus subject areas

  • General

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