Glial pathology and retinal neurotoxicity in the anterior visual pathway in experimental autoimmune encephalomyelitis

Jing Jin, Matthew D. Smith, Calvin J. Kersbergen, Tae-In Kam, Mayuri Viswanathan, Kyle Martin, Ted M Dawson, Valina Dawson, Donald J Zack, Katharine Whartenby, Peter Calabresi

Research output: Contribution to journalArticle

Abstract

The animal model experimental autoimmune encephalomyelitis (EAE) has been used extensively in the past to test mechanisms that target peripheral immune cells for treatment of multiple sclerosis (MS). While there have been some notable successes in relapsing MS, the development of therapies for progressive multiple sclerosis (MS) has been hampered by lack of an appropriate animal model. Further, the mechanisms underlying CNS inflammation and neuronal injury remain incompletely elucidated. It is known that the MOG 35-55 EAE mouse model does not have insidious behavioral progression as occurs in people with MS, but there is significant neuronal and axonal injury in EAE, as a result of the inflammation. In the present study, we describe the time course of glial activation and retinal neurodegeneration in the EAE model, and highlight the utility of studying the anterior visual pathway for modeling mechanisms of neuronal injury that may recapitulate critical aspects of the pathology described in people with MS following optic neuritis and subclinical optic neuropathy. We show that A1 neurotoxic astrocytes are prevalent in optic nerve tissue and retina, and are associated with subsequent RGC loss in the most commonly used form of the EAE model induced by MOG 35-55 peptide in C57/B6 mice. We developed a semi-automatic method to quantify retinal ganglion cells (RGC) and show that RGCs remain intact at peak EAE (PID 16) but are significantly reduced in late EAE (PID 42). Postsynaptic proteins and neurites were also compromised in the retina of late EAE mice. The retinal pathology manifests weeks after the microglial and astrocyte activation, which were prominent in optic nerve tissues at PID 16. Microglia expressed iNOS and had increased gene expression of C1q, TNF-α, and IL-1α. Astrocytes expressed high levels of complement component 3 and other genes associated with A1 neurotoxic astrocytes. Our data suggest that EAE can be used to study the pathobiology of optic neuropathy and to examine the preclinical neuroprotective effects of drugs that target activation of neurotoxic A1 astrocytes.

Original languageEnglish (US)
Number of pages1
JournalActa Neuropathologica Communications
Volume7
Issue number1
DOIs
StatePublished - Jul 31 2019

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Visual Pathways
Autoimmune Experimental Encephalomyelitis
Neuroglia
Pathology
Astrocytes
Multiple Sclerosis
Nerve Tissue
Optic Nerve Diseases
Retinal Ganglion Cells
Neuroprotective Agents
Optic Nerve
Retina
Wounds and Injuries
Animal Models
Inflammation
Optic Neuritis
Complement C3
Microglia
Neurites
Interleukin-1

Keywords

  • Astrocytes
  • EAE
  • Optic neuritis
  • Retinal ganglion cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Glial pathology and retinal neurotoxicity in the anterior visual pathway in experimental autoimmune encephalomyelitis. / Jin, Jing; Smith, Matthew D.; Kersbergen, Calvin J.; Kam, Tae-In; Viswanathan, Mayuri; Martin, Kyle; Dawson, Ted M; Dawson, Valina; Zack, Donald J; Whartenby, Katharine; Calabresi, Peter.

In: Acta Neuropathologica Communications, Vol. 7, No. 1, 31.07.2019.

Research output: Contribution to journalArticle

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