Glial glutamate transporter GLT-1 down-regulation precedes delayed neuronal death in gerbil hippocampus following transient global cerebral ischemia

Vemuganti L. Raghavendra Rao, Adibhatla Muralikrishna Rao, Aclan Dogan, Kellie K. Bowen, James Hatcher, Jeffrey D Rothstein, Robert J. Dempsey

Research output: Contribution to journalArticle

Abstract

Glial (GLT-1 and GLAST) and neuronal (EAAC1) high-affinity transporters mediate the sodium dependent glutamate reuptake in mammalian brain. Their dysfunction leads to neuronal damage by allowing glutamate to remain in the synaptic cleft for a longer duration. The purpose of the present study is to understand their contribution to the ischemic delayed neuronal death seen in gerbil hippocampus following transient global cerebral ischemia. The protein levels of these three transporters were studied by immunoblotting as a function of reperfusion time (6 h to 7 days) following a 10 min occlusion of bilateral common carotid arteries in gerbils. In the vulnerable hippocampus, there was a significant decrease in the protein levels of GLT-1 (by 36-46%, P

Original languageEnglish (US)
Pages (from-to)531-537
Number of pages7
JournalNeurochemistry International
Volume36
Issue number6
DOIs
StatePublished - May 2000

Fingerprint

Amino Acid Transport System X-AG
Gerbillinae
Transient Ischemic Attack
Neuroglia
Hippocampus
Down-Regulation
Sodium Glutamate
Common Carotid Artery
Immunoblotting
Reperfusion
Glutamic Acid
Proteins
Brain

Keywords

  • Delayed neuronal death
  • Gerbil
  • Global cerebral Ischemia
  • Glutamate transporters
  • Hippocampus
  • Immunoblotting

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Glial glutamate transporter GLT-1 down-regulation precedes delayed neuronal death in gerbil hippocampus following transient global cerebral ischemia. / Raghavendra Rao, Vemuganti L.; Muralikrishna Rao, Adibhatla; Dogan, Aclan; Bowen, Kellie K.; Hatcher, James; Rothstein, Jeffrey D; Dempsey, Robert J.

In: Neurochemistry International, Vol. 36, No. 6, 05.2000, p. 531-537.

Research output: Contribution to journalArticle

Raghavendra Rao, Vemuganti L. ; Muralikrishna Rao, Adibhatla ; Dogan, Aclan ; Bowen, Kellie K. ; Hatcher, James ; Rothstein, Jeffrey D ; Dempsey, Robert J. / Glial glutamate transporter GLT-1 down-regulation precedes delayed neuronal death in gerbil hippocampus following transient global cerebral ischemia. In: Neurochemistry International. 2000 ; Vol. 36, No. 6. pp. 531-537.
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