Giant vesicles functionally expressing membrane receptors for an insect pheromone

Satoshi Hamada, Masashi Tabuchi, Taro Toyota, Takeshi Sakurai, Tomohiro Hosoi, Tomonori Nomoto, Kei Nakatani, Masanori Fujinami, Ryohei Kanzaki

Research output: Contribution to journalArticle

Abstract

To date, biochemical approaches to membrane receptors have been limited to the following methods: knockout or overexpression of membrane receptors by gene introduction and genome engineering or extraction of membrane receptor-surfactant complexes from innate cells and their introduction into model biomembranes. Here, we describe the development of a third method involving gene expression using cell-free in situ protein synthesis inside model biomembrane capsules. We verified this method by synthesizing olfactory receptors from the silkmoth Bombyx mori inside giant vesicles and found that they were excited in the presence of their ligand the Bombyx mori sex pheromone.

Original languageEnglish (US)
Pages (from-to)2958-2961
Number of pages4
JournalChemical Communications
Volume50
Issue number22
DOIs
StatePublished - Mar 18 2014
Externally publishedYes

Fingerprint

Pheromones
Membranes
Genes
Odorant Receptors
Sex Attractants
Gene expression
Surface-Active Agents
Capsules
Surface active agents
Ligands
Proteins

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Hamada, S., Tabuchi, M., Toyota, T., Sakurai, T., Hosoi, T., Nomoto, T., ... Kanzaki, R. (2014). Giant vesicles functionally expressing membrane receptors for an insect pheromone. Chemical Communications, 50(22), 2958-2961. https://doi.org/10.1039/c3cc48216b

Giant vesicles functionally expressing membrane receptors for an insect pheromone. / Hamada, Satoshi; Tabuchi, Masashi; Toyota, Taro; Sakurai, Takeshi; Hosoi, Tomohiro; Nomoto, Tomonori; Nakatani, Kei; Fujinami, Masanori; Kanzaki, Ryohei.

In: Chemical Communications, Vol. 50, No. 22, 18.03.2014, p. 2958-2961.

Research output: Contribution to journalArticle

Hamada, S, Tabuchi, M, Toyota, T, Sakurai, T, Hosoi, T, Nomoto, T, Nakatani, K, Fujinami, M & Kanzaki, R 2014, 'Giant vesicles functionally expressing membrane receptors for an insect pheromone', Chemical Communications, vol. 50, no. 22, pp. 2958-2961. https://doi.org/10.1039/c3cc48216b
Hamada, Satoshi ; Tabuchi, Masashi ; Toyota, Taro ; Sakurai, Takeshi ; Hosoi, Tomohiro ; Nomoto, Tomonori ; Nakatani, Kei ; Fujinami, Masanori ; Kanzaki, Ryohei. / Giant vesicles functionally expressing membrane receptors for an insect pheromone. In: Chemical Communications. 2014 ; Vol. 50, No. 22. pp. 2958-2961.
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