Abstract
To date, biochemical approaches to membrane receptors have been limited to the following methods: knockout or overexpression of membrane receptors by gene introduction and genome engineering or extraction of membrane receptor-surfactant complexes from innate cells and their introduction into model biomembranes. Here, we describe the development of a third method involving gene expression using cell-free in situ protein synthesis inside model biomembrane capsules. We verified this method by synthesizing olfactory receptors from the silkmoth Bombyx mori inside giant vesicles and found that they were excited in the presence of their ligand the Bombyx mori sex pheromone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2958-2961 |
| Number of pages | 4 |
| Journal | Chemical Communications |
| Volume | 50 |
| Issue number | 22 |
| DOIs | |
| State | Published - Mar 18 2014 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Catalysis
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Chemistry(all)
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry
Cite this
Giant vesicles functionally expressing membrane receptors for an insect pheromone. / Hamada, Satoshi; Tabuchi, Masashi; Toyota, Taro; Sakurai, Takeshi; Hosoi, Tomohiro; Nomoto, Tomonori; Nakatani, Kei; Fujinami, Masanori; Kanzaki, Ryohei.
In: Chemical Communications, Vol. 50, No. 22, 18.03.2014, p. 2958-2961.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Giant vesicles functionally expressing membrane receptors for an insect pheromone
AU - Hamada, Satoshi
AU - Tabuchi, Masashi
AU - Toyota, Taro
AU - Sakurai, Takeshi
AU - Hosoi, Tomohiro
AU - Nomoto, Tomonori
AU - Nakatani, Kei
AU - Fujinami, Masanori
AU - Kanzaki, Ryohei
PY - 2014/3/18
Y1 - 2014/3/18
N2 - To date, biochemical approaches to membrane receptors have been limited to the following methods: knockout or overexpression of membrane receptors by gene introduction and genome engineering or extraction of membrane receptor-surfactant complexes from innate cells and their introduction into model biomembranes. Here, we describe the development of a third method involving gene expression using cell-free in situ protein synthesis inside model biomembrane capsules. We verified this method by synthesizing olfactory receptors from the silkmoth Bombyx mori inside giant vesicles and found that they were excited in the presence of their ligand the Bombyx mori sex pheromone.
AB - To date, biochemical approaches to membrane receptors have been limited to the following methods: knockout or overexpression of membrane receptors by gene introduction and genome engineering or extraction of membrane receptor-surfactant complexes from innate cells and their introduction into model biomembranes. Here, we describe the development of a third method involving gene expression using cell-free in situ protein synthesis inside model biomembrane capsules. We verified this method by synthesizing olfactory receptors from the silkmoth Bombyx mori inside giant vesicles and found that they were excited in the presence of their ligand the Bombyx mori sex pheromone.
UR - http://www.scopus.com/inward/record.url?scp=84894443581&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84894443581&partnerID=8YFLogxK
U2 - 10.1039/c3cc48216b
DO - 10.1039/c3cc48216b
M3 - Article
C2 - 24509495
AN - SCOPUS:84894443581
VL - 50
SP - 2958
EP - 2961
JO - Chemical Communications
JF - Chemical Communications
SN - 1359-7345
IS - 22
ER -