Abstract
Chloride channels supply critical functions in epithelial cells throughout the body. Although function of the volume- and voltage-gated ClC- 2 is uncertain, its wide tissue distribution of mRNA suggests ClC-2 has important housekeeping functions. This study's objective was to identify the extent of not only ClC-2 mRNA expression but also protein expression as a measure of the capacity for ClC-2 chloride secretion in epithelial tissues. Using quantitative ribonuclease protection assay, we found that ClC-2 mRNA transcripts were abundant in fetal and postnatal brain, fetal kidney, liver, intestine, and lung. In contrast to brain, ClC-2 mRNA transcripts were downregulated during late gestation in lung, kidney, and intestine. The lung expressed the least ClC-2 mRNA. Immunoblotting demonstrated similar tissue- and gestation-dependent variations in ClC-2 protein expression. To determine if there is a correlation between the sites of ClC-2 protein expression and cystic fibrosis transmembrane conductance regulator (CFTR), another epithelial chloride channel, a polyclonal COOH-terminal ClC-2 antibody and an anti-R domain CFTR antibody were used. ClC-2 and CFTR were expressed in different sites in lung and kidney.
Original language | English (US) |
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Pages (from-to) | L829-L837 |
Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
Volume | 271 |
Issue number | 5 15-5 |
DOIs | |
State | Published - Nov 1996 |
Externally published | Yes |
Keywords
- cystic fibrosis transmembrane conductance regulator
- fetal development
- intestine
- kidney
- lung
ASJC Scopus subject areas
- Physiology
- Pulmonary and Respiratory Medicine
- Physiology (medical)
- Cell Biology