Gestational and tissue-specific regulation of ClC-2 chloride channel expression

Carol B. Murray, Shijian Chu, Pamela L. Zeitlin

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


Chloride channels supply critical functions in epithelial cells throughout the body. Although function of the volume- and voltage-gated ClC- 2 is uncertain, its wide tissue distribution of mRNA suggests ClC-2 has important housekeeping functions. This study's objective was to identify the extent of not only ClC-2 mRNA expression but also protein expression as a measure of the capacity for ClC-2 chloride secretion in epithelial tissues. Using quantitative ribonuclease protection assay, we found that ClC-2 mRNA transcripts were abundant in fetal and postnatal brain, fetal kidney, liver, intestine, and lung. In contrast to brain, ClC-2 mRNA transcripts were downregulated during late gestation in lung, kidney, and intestine. The lung expressed the least ClC-2 mRNA. Immunoblotting demonstrated similar tissue- and gestation-dependent variations in ClC-2 protein expression. To determine if there is a correlation between the sites of ClC-2 protein expression and cystic fibrosis transmembrane conductance regulator (CFTR), another epithelial chloride channel, a polyclonal COOH-terminal ClC-2 antibody and an anti-R domain CFTR antibody were used. ClC-2 and CFTR were expressed in different sites in lung and kidney.

Original languageEnglish (US)
Pages (from-to)L829-L837
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number5 15-5
StatePublished - Nov 1996


  • cystic fibrosis transmembrane conductance regulator
  • fetal development
  • intestine
  • kidney
  • lung

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology


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