Germ cell specification

Jennifer T. Wang, Geraldine Seydoux

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The germline of Caenorhabditis elegans derives from a single founder cell, the germline blastomere P4. P4 is the product of four asymmetric cleavages that divide the zygote into distinct somatic and germline (P) lineages. P4 inherits a specialized cytoplasm (germ plasm) containing maternally encoded proteins and RNAs. The germ plasm has been hypothesized to specify germ cell fate, but the mechanisms involved remain unclear. Three processes stand out: (1) inhibition of mRNA transcription to prevent activation of somatic development, (2) translational regulation of the nanos homolog nos-2 and of other germ plasm mRNAs, and (3) establishment of a unique, partially repressive chromatin. Together, these processes ensure that the daughters of P4, the primordial germ cells Z2 and Z3, gastrulate inside the embryo, associate with the somatic gonad, initiate the germline transcriptional program, and proliferate during larval development to generate ∼2,000 germ cells by adulthood.

Original languageEnglish (US)
Title of host publicationGerm Cell Development in C. elegans
PublisherSpringer Science and Business Media, LLC
Pages17-39
Number of pages23
ISBN (Print)9781461440147
DOIs
StatePublished - 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume757
ISSN (Print)0065-2598

Keywords

  • Cell fate
  • Germ granules
  • Germ plasm
  • Germline blastomeres
  • Maternal RNA
  • P lineage
  • Polarity
  • Primordial germ cells
  • Transcriptional repression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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