Germ cell-Sertoli cell interactions: Regulation by germ cells of the stage-specific expression of CP-2/cathepsin L mRNA by Sertoli cells

William W Wright, S. D. Zabludoff, T. L. Penttila, M. Parvinen

Research output: Contribution to journalArticle

Abstract

CP-2/cathepsin L mRNA is expressed primarily by, rat Sertoli cells within stage VI-VIII seminiferous tubules. To test whether germ cells regulated this expression, we examined if separating Sertoli cells from specific germ cells affected expression of this transcript in Sertoli cells. First, Sertoli cells were isolated from adult (90-day-old) and immature (25 day old) rats and levels of this transcript measured immediately or after 1,3 and 5 days in culture. Results demonstrated that immediately upon isolation, CP- 2/cathepsin L mRNA levels were significantly higher in mature cells. However, after 1 day in culture, the levels of this transcript increased in immature cells and remained high in mature cells. We therefore conclude that in vivo, a subset of germ cells inhibit the expression of CP-2/cathepsin L mRNA by immature Sertoli cells. Second, to examine the effect of specific germ cells on CP-2/cathepsin L mRNA expression, we exposed the testes of mature rats to 3 Gy of γ-radiation and analyzed stage-specific expression of this transcript at varying times during maturation depletion and subsequent germ cell restoration. Loss of spermatogonia or spermatocytes was without effect. However, when pachytene spermatocytes through step 14 spermatids were depleted, expression at stages VI-VIII was reduced by half and expression at stages IX-I was increased 14-fold. These changes resulted in the loss of stage-specific expression of CP-2/cathepsin L mRNA by Sertoli cells. Finally, stage VI-VIII tubules, depleted primarily in step 15-19 spermatids, had levels of CP-2/cathepsin L mRNA that were 60% of control. However, stage- specific expression of this transcript was detected in these tubules. In contrast to what we noted with CP-2/cathepsin L mRNA, loss and restoration of germ cells had no effect on Sertoli cell levels of SGP-2 mRNA, indicating that testicular irradiation had no overall effect on Sertoli cell function. Taken together, these data suggest that the stage-specific expression of the CP-2/cathepsin L gene results from the sequential stimulation and inhibition of Sertoli cells by germ cells, that pachytene spermatocytes through step 14 spermatids are required for this stage-specific expression and that step 18 and 19 spermatids amplify this expression at stages VI-VIII.

Original languageEnglish (US)
Pages (from-to)104-113
Number of pages10
JournalDevelopmental Genetics
Volume16
Issue number2
DOIs
StatePublished - 1995

Fingerprint

Cathepsin L
Sertoli Cells
Germ Cells
Cell Communication
Messenger RNA
Spermatids
Spermatocytes
Radiation Dosage
Spermatogonia
Seminiferous Tubules
Testis

Keywords

  • CP-2/cathepsin L
  • germ cells
  • Sertoli cells
  • Spermatogenesis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics

Cite this

Germ cell-Sertoli cell interactions : Regulation by germ cells of the stage-specific expression of CP-2/cathepsin L mRNA by Sertoli cells. / Wright, William W; Zabludoff, S. D.; Penttila, T. L.; Parvinen, M.

In: Developmental Genetics, Vol. 16, No. 2, 1995, p. 104-113.

Research output: Contribution to journalArticle

@article{774bfcfe771b483a8a868e3d0fe95f8d,
title = "Germ cell-Sertoli cell interactions: Regulation by germ cells of the stage-specific expression of CP-2/cathepsin L mRNA by Sertoli cells",
abstract = "CP-2/cathepsin L mRNA is expressed primarily by, rat Sertoli cells within stage VI-VIII seminiferous tubules. To test whether germ cells regulated this expression, we examined if separating Sertoli cells from specific germ cells affected expression of this transcript in Sertoli cells. First, Sertoli cells were isolated from adult (90-day-old) and immature (25 day old) rats and levels of this transcript measured immediately or after 1,3 and 5 days in culture. Results demonstrated that immediately upon isolation, CP- 2/cathepsin L mRNA levels were significantly higher in mature cells. However, after 1 day in culture, the levels of this transcript increased in immature cells and remained high in mature cells. We therefore conclude that in vivo, a subset of germ cells inhibit the expression of CP-2/cathepsin L mRNA by immature Sertoli cells. Second, to examine the effect of specific germ cells on CP-2/cathepsin L mRNA expression, we exposed the testes of mature rats to 3 Gy of γ-radiation and analyzed stage-specific expression of this transcript at varying times during maturation depletion and subsequent germ cell restoration. Loss of spermatogonia or spermatocytes was without effect. However, when pachytene spermatocytes through step 14 spermatids were depleted, expression at stages VI-VIII was reduced by half and expression at stages IX-I was increased 14-fold. These changes resulted in the loss of stage-specific expression of CP-2/cathepsin L mRNA by Sertoli cells. Finally, stage VI-VIII tubules, depleted primarily in step 15-19 spermatids, had levels of CP-2/cathepsin L mRNA that were 60{\%} of control. However, stage- specific expression of this transcript was detected in these tubules. In contrast to what we noted with CP-2/cathepsin L mRNA, loss and restoration of germ cells had no effect on Sertoli cell levels of SGP-2 mRNA, indicating that testicular irradiation had no overall effect on Sertoli cell function. Taken together, these data suggest that the stage-specific expression of the CP-2/cathepsin L gene results from the sequential stimulation and inhibition of Sertoli cells by germ cells, that pachytene spermatocytes through step 14 spermatids are required for this stage-specific expression and that step 18 and 19 spermatids amplify this expression at stages VI-VIII.",
keywords = "CP-2/cathepsin L, germ cells, Sertoli cells, Spermatogenesis",
author = "Wright, {William W} and Zabludoff, {S. D.} and Penttila, {T. L.} and M. Parvinen",
year = "1995",
doi = "10.1002/dvg.1020160203",
language = "English (US)",
volume = "16",
pages = "104--113",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Germ cell-Sertoli cell interactions

T2 - Regulation by germ cells of the stage-specific expression of CP-2/cathepsin L mRNA by Sertoli cells

AU - Wright, William W

AU - Zabludoff, S. D.

AU - Penttila, T. L.

AU - Parvinen, M.

PY - 1995

Y1 - 1995

N2 - CP-2/cathepsin L mRNA is expressed primarily by, rat Sertoli cells within stage VI-VIII seminiferous tubules. To test whether germ cells regulated this expression, we examined if separating Sertoli cells from specific germ cells affected expression of this transcript in Sertoli cells. First, Sertoli cells were isolated from adult (90-day-old) and immature (25 day old) rats and levels of this transcript measured immediately or after 1,3 and 5 days in culture. Results demonstrated that immediately upon isolation, CP- 2/cathepsin L mRNA levels were significantly higher in mature cells. However, after 1 day in culture, the levels of this transcript increased in immature cells and remained high in mature cells. We therefore conclude that in vivo, a subset of germ cells inhibit the expression of CP-2/cathepsin L mRNA by immature Sertoli cells. Second, to examine the effect of specific germ cells on CP-2/cathepsin L mRNA expression, we exposed the testes of mature rats to 3 Gy of γ-radiation and analyzed stage-specific expression of this transcript at varying times during maturation depletion and subsequent germ cell restoration. Loss of spermatogonia or spermatocytes was without effect. However, when pachytene spermatocytes through step 14 spermatids were depleted, expression at stages VI-VIII was reduced by half and expression at stages IX-I was increased 14-fold. These changes resulted in the loss of stage-specific expression of CP-2/cathepsin L mRNA by Sertoli cells. Finally, stage VI-VIII tubules, depleted primarily in step 15-19 spermatids, had levels of CP-2/cathepsin L mRNA that were 60% of control. However, stage- specific expression of this transcript was detected in these tubules. In contrast to what we noted with CP-2/cathepsin L mRNA, loss and restoration of germ cells had no effect on Sertoli cell levels of SGP-2 mRNA, indicating that testicular irradiation had no overall effect on Sertoli cell function. Taken together, these data suggest that the stage-specific expression of the CP-2/cathepsin L gene results from the sequential stimulation and inhibition of Sertoli cells by germ cells, that pachytene spermatocytes through step 14 spermatids are required for this stage-specific expression and that step 18 and 19 spermatids amplify this expression at stages VI-VIII.

AB - CP-2/cathepsin L mRNA is expressed primarily by, rat Sertoli cells within stage VI-VIII seminiferous tubules. To test whether germ cells regulated this expression, we examined if separating Sertoli cells from specific germ cells affected expression of this transcript in Sertoli cells. First, Sertoli cells were isolated from adult (90-day-old) and immature (25 day old) rats and levels of this transcript measured immediately or after 1,3 and 5 days in culture. Results demonstrated that immediately upon isolation, CP- 2/cathepsin L mRNA levels were significantly higher in mature cells. However, after 1 day in culture, the levels of this transcript increased in immature cells and remained high in mature cells. We therefore conclude that in vivo, a subset of germ cells inhibit the expression of CP-2/cathepsin L mRNA by immature Sertoli cells. Second, to examine the effect of specific germ cells on CP-2/cathepsin L mRNA expression, we exposed the testes of mature rats to 3 Gy of γ-radiation and analyzed stage-specific expression of this transcript at varying times during maturation depletion and subsequent germ cell restoration. Loss of spermatogonia or spermatocytes was without effect. However, when pachytene spermatocytes through step 14 spermatids were depleted, expression at stages VI-VIII was reduced by half and expression at stages IX-I was increased 14-fold. These changes resulted in the loss of stage-specific expression of CP-2/cathepsin L mRNA by Sertoli cells. Finally, stage VI-VIII tubules, depleted primarily in step 15-19 spermatids, had levels of CP-2/cathepsin L mRNA that were 60% of control. However, stage- specific expression of this transcript was detected in these tubules. In contrast to what we noted with CP-2/cathepsin L mRNA, loss and restoration of germ cells had no effect on Sertoli cell levels of SGP-2 mRNA, indicating that testicular irradiation had no overall effect on Sertoli cell function. Taken together, these data suggest that the stage-specific expression of the CP-2/cathepsin L gene results from the sequential stimulation and inhibition of Sertoli cells by germ cells, that pachytene spermatocytes through step 14 spermatids are required for this stage-specific expression and that step 18 and 19 spermatids amplify this expression at stages VI-VIII.

KW - CP-2/cathepsin L

KW - germ cells

KW - Sertoli cells

KW - Spermatogenesis

UR - http://www.scopus.com/inward/record.url?scp=0028958806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028958806&partnerID=8YFLogxK

U2 - 10.1002/dvg.1020160203

DO - 10.1002/dvg.1020160203

M3 - Article

C2 - 7736660

AN - SCOPUS:0028958806

VL - 16

SP - 104

EP - 113

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 2

ER -