Gentamicin uptake by cochlear hair cells precedes hearing impairment during chronic treatment

Hakim Hiel, Jean Paul Erre, Catherine Aurousseau, Rabia Bouali, Didier Dulon, Jean Marie Aran

Research output: Contribution to journalArticle

Abstract

Immunodetection of gentamicin (GM) was carried out on surface preparations of the whole organ of Corti from cochleas of guinea pigs treated daily with GM at a dose of 60 mg/kg/day and sacrificed at the end of different treatment periods. Cochlear function was determined just before sacrifice, 24 h after the last injection. Threshold elevations, mainly at high frequencies, were noted only after 10-14 days of treatment. However, the presence of GM was observed much earlier, as early as after the second injection, and specifically in the sensory hair cells. GM labelling was essentially observed in the outer hair cells (OHC) and increased from the apex to the base of the cochlea and from the third to the first row of OHC. GM labelling of inner hair cells was less pronounced and was observed only after the 8th day of treatment. These observations demonstrate that GM specifically enters and accumulates in the sensory hair cells and that the uptake precedes the development of functional and cellular damage which may result from a long-term intracellular cytotoxic action of the molecule.

Original languageEnglish (US)
Pages (from-to)78-87
Number of pages10
JournalInternational Journal of Audiology
Volume32
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Keywords

  • Aminoglycoside antibiotics
  • Auditory-evoked potentials
  • Hair cells
  • Immunohistochemistry
  • Thresholds

ASJC Scopus subject areas

  • Language and Linguistics
  • Linguistics and Language
  • Speech and Hearing

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