Genotyping by mass spectrometric analysis of short DNA fragments

Steven J. Laken, Peta E. Jackson, Kenneth W Kinzler, Bert Vogelstein, Paul Timothy Strickland, John Davis Groopman, Marlin D. Friesen

Research output: Contribution to journalArticle

Abstract

A method has been developed to produce small DNA fragments from PCR products for analysis of defined DNA variations by mass spectrometry. The genomic region to be analyzed is PCR-amplified with primers containing a sequence for the type IIS restriction endonuclease Bpml. Bpml digestion of the resultant PCR products yields fragments as small as seven bases, which are then analyzed by electrospray ionization mass spectrometry. The approach was validated using seven different variants within the APC tumor suppressor gene, in which a perfect correlation was obtained with DNA sequencing. Both the sense and antisense strands were analyzed independently, and several variants can be analyzed simultaneously. These results provide the basis for a generally applicable and highly accurate method that directly queries the mass of variant DNA sequences.

Original languageEnglish (US)
Pages (from-to)1352-1356
Number of pages5
JournalNature Biotechnology
Volume16
Issue number13
DOIs
StatePublished - Dec 1998

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Keywords

  • Genomics
  • Single nucleotide polymorphisms
  • Type IIS restriction endonuclease

ASJC Scopus subject areas

  • Microbiology

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